ATP生物荧光增幅法在化妆品微生物检测中的适用性研究

doi:10.3969/j.issn.1001-1803.2021.06.007

Abstract

Abstract:

34 commercial cosmetics from 17 brands, covering ordinary cosmetics and special cosmetics with different functions, parts of action, formulations and users, were collected to conduct the applicability study of amplified ATP bioluminescence assay in microbial detection of cosmetics. Sample effect test was conducted firstly, and the products whose signal values met the requirements of the method were selected for further treatment. Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Candida albicans and Aspergillus niger were separately inoculated into the selected cosmetics at the target concentration of 10-100 CFU/g, the results of which were used to evaluate the applicability of amplified ATP bioluminescence assay. Results show that the signal values of 31 cosmetics are between 45-526 RLU, which are less than 3-fold signal value of medium blank and meet the requirements of the detection method. The signal values of 3 products are between 610-7 791 RLU, which are higher than 3-fold signal value of the medium blank and do not meet the method requirements. The test results of 27 products contaminated with 6 strains are all positive. 4 products are partially positively detected, and need to be further tested through dilution, neutralization, membrane filtration or other methods. In conclusion, amplified ATP bioluminescence assay is an effective and practical method for the microbial detection of cosmetics, and it can be applied in the rapid screening of the cosmetic products contaminated with microbes.

Key words: amplified ATP bioluminescence assay, cosmetics, microorganism, suitability

CLC Number:

TQ658

LI Ting,SHEN Ying,CAI Jun-song,LIU Yi-ru,CHEN Yi-wen,LIU Ji,YAO Su. Applicability study of amplified ATP bioluminescence assay in microbial detection of cosmetics[J].China Surfactant Detergent & Cosmetics, 2021, 51(6): 513-521.

Figures/Tables 4

Tab. 1

Tab. 2

Test results of sample effect"

编号	产品名称	批次	培养基空白平均信号值/RLU	微生物对照平均信号值/ 培养基空白平均信号值						ATP培养基对照平均信号值/RLU	产品样品平均信号值/RLU	平板生长情况	微生物样品平均信号值/ 培养基空白平均信号值						ATP样品平均信号值/RLU	产品ATP回收率/ %
编号	产品名称	批次	培养基空白平均信号值/RLU	黑曲霉	白色念珠菌	枯草芽胞杆菌	铜绿假单胞菌	金黄色葡萄球菌	大肠埃希氏菌	ATP培养基对照平均信号值/RLU	产品样品平均信号值/RLU	平板生长情况	黑曲霉	白色念珠菌	枯草芽胞杆菌	铜绿假单胞菌	金黄色葡萄球菌	大肠埃希氏菌	ATP样品平均信号值/RLU	产品ATP回收率/ %
1	莹泽洁面乳	Lot 1	191	P	P	P	P	P	P	7 978	45	—	P	P	P	P	P	P	775	9.38
		Lot 2									53	—	P	P	P	P	P	P	1 122	13.73
		Lot 3									65	—	P	P	P	P	P	P	1 149	13.92
2	洁面膏	Lot 1	165	P	P	P	P	P	P	15 992	330	—	P	P	P	P	P	P	3 253	26.29
		Lot 2									237	—	P	P	P	P	P	P	3 026	18.60
		Lot 3									348	—	P	P	P	P	P	P	3 228	19.53
3	氨基酸洁面泡泡	Lot 1	181	P	P	P	P	P	P	6 691	141	—	P	P	P	P	P	P	5 079	75.85
		Lot 2									170	—	P	P	P	P	P	P	4 179	61.58
		Lot 3									157	—	P	P	P	P	P	P	4 213	62.30
4	滋润洗手液	Lot 1	194	P	P	P	P	P	P	5 948	157	—	P	P	P	P	P	P	4 505	75.56
		Lot 2									324	—	P	P	P	P	P	P	4 780	77.44
		Lot 3									174	—	P	P	P	P	P	P	4 749	79.51
5	玫瑰洗手液	Lot 1	181	P	P	P	P	P	P	6 691	248	—	P	P	P	P	P	P	5 954	87.65
		Lot 2									226	—	P	P	P	P	P	P	5 779	85.30
		Lot 3									424	—	P	P	P	P	P	P	6 599	94.85
6	美肌沐浴露	Lot 1	194	P	P	P	P	P	P	5 948	212	—	P	P	P	P	P	P	5 782	96.80
		Lot 2									390	—	P	P	P	P	P	P	6 207	101.09
		Lot 3									334	—	P	P	P	P	P	P	6 402	102.02
7	香氛沐浴乳	Lot 1	167	P	P	P	P	P	P	5 539	115	—	P	P	P	P	P	P	5 045	91.77
		Lot 2									123	—	P	P	P	P	P	P	4 650	84.27
		Lot 3									118	—	P	P	P	P	P	P	4 380	76.95
8	焕活芯生洗发乳	Lot 1	194	P	P	P	P	P	P	5 948	7 791	—	P	P	P	P	P	P	13 657	101.95
		Lot 2									7 102	—	P	P	P	P	P	P	12 736	97.91
		Lot 3									7 038	—	P	P	P	P	P	P	12 703	98.45
9	清润洗发水	Lot 1	187	P	P	P	P	P	P	6 761	153	—	P	P	P	P	P	P	4 484	65.88
		Lot 2									174	—	P	P	P	P	P	P	5 225	76.83
		Lot 3									248	—	P	P	P	P	P	P	5 050	71.02
10	修护洗发露	Lot 1	285	P	P	P	P	P	P	6 060	157	—	P	P	P	P	P	P	4 416	73.75
		Lot 2									87	—	P	P	P	P	P	P	4 624	78.56
		Lot 3									182	—	P	P	P	P	P	P	4 490	74.60
11	祛屑舒爽洗发乳液	Lot 1	212	P	P	P	P	P	P	3 721	136	—	P	P	P	P	P	P	4 811	133.23
		Lot 2									170	—	P	P	P	P	P	P	4 500	123.40
		Lot 3									138	—	P	P	P	P	P	P	4 925	128.65
12	剃须泡	Lot 1	165	P	P	P	P	P	P	15 992	373	—	P	P	P	P	P	P	9 126	55.30
		Lot 2									144	—	P	P	P	P	P	P	9 627	59.92
		Lot 3									255	—	P	P	P	P	P	P	6 898	41.54
13	胶原蛋白弹润面膜	Lot 1	193	P	P	P	P	P	P	7 978	257	—	P	P	P	P	P	P	6 479	79.92
		Lot 2									223	—	P	P	P	P	P	P	5 147	63.25
		Lot 3									245	—	P	P	P	P	P	P	5 912	71.03
14	水感透白光塑钻纹弹力面膜	Lot 1	167	P	P	P	P	P	P	5 539	170	—	P	P	P	P	P	P	5 833	105.42
		Lot 2									198	—	P	P	P	P	P	P	5 937	106.83
		Lot 3									166	—	P	P	P	P	P	P	5 818	105.21
15	倍护精华修护润肤露	Lot 1	165	P	P	P	P	P	P	15 992	349	—	P	P	P	P	P	P	6 307	37.64
		Lot 2									329	—	P	P	P	P	P	P	6 415	38.45
		Lot 3									271	—	P	P	P	P	P	P	6 336	37.93
16	新生塑颜金纯面霜	Lot 1	199	P	P	P	P	P	P	6 242	182	—	P	P	P	P	P	P	6 112	98.13
		Lot 2									199	—	P	P	P	P	P	P	6 080	97.32
		Lot 3									182	—	P	P	P	P	P	P	6 082	97.63
17	护手霜	Lot 1	165	P	P	P	P	P	P	15 992	210	—	P	P	P	P	P	P	6 123	37.36
		Lot 2									151	—	P	P	P	P	P	P	5 939	36.57
		Lot 3									287	—	P	P	P	P	P	P	6 727	40.69
18	塑颜新肌紧致精华水	Lot 1	193	P	P	P	P	P	P	7 978	286	—	P	P	P	P	P	P	5 262	63.92
		Lot 2									201	—	P	P	P	P	P	P	5 324	65.81
		Lot 3									359	—	P	P	P	P	P	P	5 106	59.50
19	水感透白光塑精华露	Lot 1	193	P	P	P	P	P	P	7 978	293	—	P	P	P	P	P	P	5 100	61.75
		Lot 2									380	—	P	P	P	P	P	P	5 701	68.35
		Lot 3									206	—	P	P	P	P	P	P	4 876	58.54
20	婴儿清凉爽身粉	Lot 1	165	P	P	P	P	P	P	15 992	183	—	P	P	P	P	P	P	11 157	69.34
		Lot 2									202	—	P	P	P	P	P	P	7 564	46.52
		Lot 3									220	—	P	P	P	P	P	P	6 299	38.41
21	婴儿玉米爽身粉	Lot 1	212	P	P	P	P	P	P	3 721	4 917	—	P	P	P	P	P	P	10 537	160.16
		Lot 2									3 038	—	P	P	P	P	P	P	8 767	163.27
		Lot 3									270	—	P	P	P	P	P	P	6 001	163.32
22	滋养水润润发精华素	Lot 1	167	P	P	P	P	P	P	5 539	172	—	P	P	P	P	P	P	5 490	98.99
		Lot 2									159	—	P	P	P	P	P	P	5 824	105.45
		Lot 3									146	—	P	P	P	P	P	P	5 770	104.69
23	植萃精华护发素	Lot 1	181	P	P	P	P	P	P	6 691	206	—	P	P	P	P	P	P	5 684	84.15
		Lot 2									253	—	P	P	P	P	P	P	6 723	99.39
		Lot 3									274	—	P	P	P	P	P	P	6 240	89.16
24	修护型润唇膏薄荷味	Lot 1	193	P	P	P	P	P	P	7 978	158	—	P	P	P	P	P	P	5 011	62.34
		Lot 2									202	—	P	P	P	P	P	P	5 779	71.64
		Lot 3									194	—	P	P	P	P	P	P	5 125	63.34
25	凝润滋养护唇膏	Lot 1	193	P	P	P	P	P	P	7 978	330	—	P	P	P	P	P	P	5 958	72.29
		Lot 2									237	—	P	P	P	P	P	P	4 881	59.65
		Lot 3									348	—	P	P	P	P	P	P	4 944	57.61
26	控油矿物质散粉	Lot 1	193	P	P	P	P	P	P	7 978	412	—	P	P	P	P	P	P	5 446	64.66
		Lot 2									526	—	P	P	P	P	P	P	6 899	81.86
		Lot 3									473	—	P	P	P	P	P	P	5 253	61.40
27	爽身气雾	Lot 1	193	P	P	P	P	P	P	7 978	222	—	P	P	P	P	P	P	6 645	82.50
		Lot 2									262	—	P	P	P	P	P	P	5 100	62.15
		Lot 3									164	—	P	P	P	P	P	P	3 262	39.79
28	强力速型保湿性定型喷雾	Lot 1	165	P	P	P	P	P	P	15 992	153	—	P	P	P	P	P	P	6 726	41.53
		Lot 2									200	—	P	P	P	P	P	P	6 590	40.37
		Lot 3									310	—	P	P	P	P	P	P	6 717	40.48
29	臻研修护防热护发喷雾	Lot 1	253	P	P	P	P	P	P	6 012	161	—	P	P	P	P	P	P	6 197	104.81
		Lot 2									140	—	P	P	P	P	P	P	5 601	94.83
		Lot 3									142	—	P	P	P	P	P	P	5 482	92.72
30	强力定型啫喱水	Lot 1	193	P	P	P	P	P	P	7 978	213	—	P	P	P	P	P	P	5 301	65.36
		Lot 2									214	—	P	P	P	P	P	P	5 616	69.39
		Lot 3									140	—	P	P	P	P	P	P	5 338	66.77
31	草本水晶牙膏	Lot 1	193	P	P	P	P	P	P	7 978	182	—	P	P	P	P	P	P	5 237	64.93
		Lot 2									216	—	P	P	P	P	P	P	4 496	54.98
		Lot 3									259	—	P	P	P	P	P	P	6 707	82.83
32	多效修护防晒霜SPF15P++	Lot 1	193	P	P	P	P	P	P	7 978	144	—	P	P	P	P	P	P	3 796	46.91
		Lot 2									107	—	P	P	P	P	P	P	3 824	47.75
		Lot 3									115	—	P	P	P	P	P	P	3 859	48.09
33	舒缓细肤倍护防晒乳SPF35P++	Lot 1	187	P	P	P	P	P	P	6 761	610	—	P	P	P	P	P	P	5 375	72.48
		Lot 2									3 040	—	P	P	P	P	P	P	7 550	68.60
		Lot 3									1 184	—	P	P	P	P	P	P	5 850	70.98
34	防晒乳 SPF50 PA+++	Lot 1	165	P	P	P	P	P	P	15 992	158	—	P	P	P	P	P	P	5 926	36.44
		Lot 2									157	—	P	P	P	P	P	P	3 284	19.76
		Lot 3									224	—	P	P	P	P	P	P	3 053	17.87

Tab. 2

Tab. 3

Inoculation test results"

编号	产品名称	批次	培养基空白平均信号值/ RLU	微生物对照平均信号值/ 培养基空白平均信号值						产品样品平均信号值/RLU	平板生长情况	微生物样品的平均信号值/ 培养基空白的平均信号值
编号	产品名称	批次	培养基空白平均信号值/ RLU	黑曲霉	白色念珠菌	枯草芽胞杆菌	铜绿假单胞菌	金黄色葡萄球菌	大肠埃希氏菌	产品样品平均信号值/RLU	平板生长情况	黑曲霉	白色念珠菌	枯草芽胞杆菌	铜绿假单胞菌	金黄色葡萄球菌	大肠埃希氏菌
1	莹泽洁面乳	Lot 1	106	P	P	P	P	P	P	62	—	P	P	P	P	P	P
		Lot 2								64	—	P	P	P	P	P	P
		Lot 3								80	—	P	P	P	P	P	P
2	洁面膏	Lot 1	106	P	P	P	P	P	P	226	—	N	P	P	P	P	P
		Lot 2								121	—	N	P	P	P	P	P
		Lot 3								162	—	N	P	P	P	P	P
3	氨基酸洁面泡泡	Lot 1	191	P	P	P	P	P	P	232	—	N	N	P	P	P	P
		Lot 2								137	—	N	N	P	P	P	P
		Lot 3								117	—	P	N	P	P	P	P
4	滋润洗手液	Lot 1	222	P	P	P	P	P	P	525	—	P	P	P	P	P	P
		Lot 2								253	—	P	P	P	P	P	P
		Lot 3								224	—	P	P	P	P	P	P
5	玫瑰洗手液	Lot 1	191	P	P	P	P	P	P	270	—	P	P	P	P	P	P
		Lot 2								442	—	P	P	P	P	P	P
		Lot 3								460	—	P	P	P	P	P	P
6	美肌沐浴露	Lot 1	222	P	P	P	P	P	P	215	—	P	P	P	P	P	P
		Lot 2								277	—	P	P	P	P	P	P
		Lot 3								246	—	P	P	P	P	P	P
7	香氛沐浴乳	Lot 1	178	P	P	P	P	P	P	115	—	P	P	P	P	P	P
		Lot 2								123	—	P	P	P	P	P	P
		Lot 3								118	—	P	P	P	P	P	P
9	清润洗发水	Lot 1	146	P	P	P	P	P	P	132	—	P	P	P	P	P	P
		Lot 2								285	—	P	P	P	P	P	P
		Lot 3								182	—	P	P	P	P	P	P
10	修护洗发露	Lot 1	145	P	P	P	P	P	P	90	—	P	P	P	P	P	P
		Lot 2								86	—	P	P	P	P	P	P
		Lot 3								87	—	P	P	P	P	P	P
11	祛屑舒爽洗发乳液	Lot 1	255	P	P	P	P	P	P	149	—	N	N	N	P	N	N
		Lot 2								176	—	N	N	N	P	N	N
		Lot 3								193	—	N	N	N	P	N	N
12	剃须泡	Lot 1	255	P	P	P	P	P	P	228	—	P	P	P	P	P	P
		Lot 2								240	—	P	P	P	P	P	P
		Lot 3								218	—	P	P	P	P	P	P
13	胶原蛋白弹润面膜	Lot 1	146	P	P	P	P	P	P	142	—	P	P	P	P	P	P
		Lot 2								162	—	P	P	P	P	P	P
		Lot 3								347	—	P	P	P	P	P	P
14	水感透白光塑钻纹弹力面膜	Lot 1	178	P	P	P	P	P	P	170	—	P	P	P	P	P	P
		Lot 2								198	—	P	P	P	P	P	P
		Lot 3								166	—	P	P	P	P	P	P
15	倍护精华修护润肤露	Lot 1	222	P	P	P	P	P	P	272	—	P	P	P	P	P	P
		Lot 2								315	—	P	P	P	P	P	P
		Lot 3								328	—	P	P	P	P	P	P
16	新生塑颜金纯面霜	Lot 1	145	P	P	P	P	P	P	150	—	P	P	P	P	P	P
		Lot 2								128	—	P	P	P	P	P	P
		Lot 3								154	—	P	P	P	P	P	P
17	护手霜	Lot 1	267	P	P	P	P	P	P	326	—	P	P	P	P	P	P
		Lot 2								221	—	P	P	P	P	P	P
		Lot 3								193	—	P	P	P	P	P	P
18	塑颜新肌紧致精华水	Lot 1	267	P	P	P	P	P	P	234	—	P	P	P	P	P	P
		Lot 2								237	—	P	P	P	P	P	P
		Lot 3								226	—	P	P	P	P	P	P
19	水感透白光塑精华露	Lot 1	187	P	P	P	P	P	P	337	—	P	P	P	P	P	P
		Lot 2								525	—	P	P	P	P	P	P
		Lot 3								235	—	P	P	P	P	P	P
20	婴儿清凉爽身粉	Lot 1	222	P	P	P	P	P	P	302	—	P	P	P	P	P	P
		Lot 2								291	—	P	P	P	P	P	P
		Lot 3								591	—	P	P	P	P	P	P
22	滋养水润润发精华素	Lot 1	178	P	P	P	P	P	P	172	—	P	P	P	P	P	P
		Lot 2								159	—	P	P	P	P	P	P
		Lot 3								146	—	P	P	P	P	P	P
23	植萃精华护发素	Lot 1	191	P	P	P	P	P	P	460	—	P	P	P	P	P	P
		Lot 2								308	—	P	P	P	P	P	P
		Lot 3								278	—	P	P	P	P	P	P
24	修护型润唇膏薄荷味	Lot 1	187	P	P	P	P	P	P	251	—	P	P	P	P	P	P
		Lot 2								215	—	P	P	P	P	P	P
		Lot 3								215	—	P	P	P	P	P	P
25	凝润滋养护唇膏	Lot 1	187	P	P	P	P	P	P	230	—	P	P	P	P	P	P
		Lot 2								357	—	P	P	P	P	P	P
		Lot 3								231	—	P	P	P	P	P	P
26	控油矿物质散粉	Lot 1	222	P	P	P	P	P	P	606	—	P	P	P	P	P	P
		Lot 2								614	—	P	P	P	P	P	P
		Lot 3								639	—	P	P	P	P	P	P
27	爽身气雾	Lot 1	187	P	P	P	P	P	P	212	—	P	P	N	P	N	P
		Lot 2								192	—	P	P	N	P	P	P
		Lot 3								187	—	P	P	N	P	P	P
28	强力速型保湿性定型喷雾	Lot 1	267	P	P	P	P	P	P	387	—	P	P	P	P	P	P
		Lot 2								220	—	P	P	P	P	P	P
		Lot 3								423	—	P	P	P	P	P	P
29	臻研修护防热护发喷雾	Lot 1	145	P	P	P	P	P	P	161	—	P	P	P	P	P	P
		Lot 2								140	—	P	P	P	P	P	P
		Lot 3								142	—	P	P	P	P	P	P
30	强力定型啫喱水	Lot 1	267	P	P	P	P	P	P	226	—	P	P	P	P	P	P
		Lot 2								258	—	P	P	P	P	P	P
		Lot 3								205	—	P	P	P	P	P	P
31	草本水晶牙膏	Lot 1	255	P	P	P	P	P	P	268	—	P	P	P	P	P	P
		Lot 2								280	—	P	P	P	P	P	P
		Lot 3								267	—	P	P	P	P	P	P
32	多效修护防晒霜SPF15P++	Lot 1	255	P	P	P	P	P	P	203	—	P	P	P	P	P	P
		Lot 2								208	—	P	P	P	P	P	P
		Lot 3								191	—	P	P	P	P	P	P
34	防晒乳SPF50 PA+++	Lot 1	106	P	P	P	P	P	P	233	—	P	P	P	P	P	P
		Lot 2								268	—	P	P	P	P	P	P
		Lot 3								257	—	P	P	P	P	P	P

Tab. 3

Tab. 4

References 12

[1]	Li Qingbin, Zhao Xiaojun, Wang Xiang, et al. Status and analysis of microbial contamination of cosmetics[J]. Chinese Journal of Health Laboratory Technology, 2006,16(2): 245-247.
[2]	Jiang Zhijie, Gao Chun, Jing Liangyi. Study on applicability of microbial test method of hair cosmetics[J]. Journal of Environment and Health, 2014,31(12): 1096-1099.
[3]	Ministry of Health of the People’s Republic of China. Hygienic standard for cosmetics[S]. Beijing:Ministry of Health of the People’s Republic of China, 2015.
[4]	Scott Spaeth, Quynh Tran, Zhuyun Liu. Evaluation of an ATP-bioluminescence rapid microbial screening method for in-process biologics[J]. PDA Journal of Pharmaceutical Science and Technology, 2018,72(6): 574-583. doi: 10.5731/pdajpst.2018.008557 pmid: 29954921
[5]	Zhai Lei, Ge Yuanyuan, Hong haijun, Liu Jiquan, et al. Feasibility study on the amplified ATP bioluminescence assay in microbial detection- Application in cosmetics field [OL/J]. Food and Fermentation Industries, 2020. DOI: 10.13995/j.cnki.11-1802/ts.025092（https://kns.cnki.net/KCMS/detail/11.1802.TS.20200728.1114.002.html）.
[6]	Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia[S]. Beijing: China Medical Science Press Volume IV, 2020.
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[8]	Liu Ruina, Sun Sijia, Ge Yuanyuan, et al. Validation study of amplified ATP bioluminescence assay in the detection of control bacteria- Application in cosmetics field [J]. Food and Fermentation Industries. https://doi.org/10.13995/j.cnki.11-1802/ts.026691
[9]	Lu Fuping, Yan Qingsong. Rapid microbiological detection of cosmetic products- ATP bioluminescence assay[J]. China Surfactant Detergent & Cosmetics, 2004 (5): 35-36.
[10]	Sun Shengpin, Wang Qunlin. Study on the applicability of microbiological testing methods for hair cosmetics[J]. For All Health, 2016,10(12): 84.
[11]	Yu Kejiao, Chen Xiaohui, Bi Kaishun. Determination of volatile oil content and bacteriostasis in ulagra[J]. Northwest Pharmaceutical Journal, 2005,20(5): 204-206.
[12]	Huang Jianwen, Fang Lingdan, Xu Kunhua, et al. Study on bacteriostatic properties of sodium lauryl alanine [J]. China Academic Journal Electronic Publishing House, 2019 (4): 24-28.

类别	功能	部位	产品种类	产品名称
普通化妆品	清洁类	皮肤	洗面奶	莹泽洁面乳、洁面膏、氨基酸洁面泡泡
			洗手液	滋润洗手液、玫瑰洗手液
			沐浴液	美肌沐浴露、香氛沐浴露
		毛发	洗发乳	焕活芯生洗发乳、清润洗发水、修护洗发露、祛屑舒爽洗发乳液
		毛发	剃须膏	剃须泡
	护理类	皮肤	面膜	胶原蛋白弹润面膜、水感透白光塑钻纹弹力面膜
			护手霜/护肤乳	倍护精华修护润肤露、新生塑颜金纯面霜、护手霜
			化妆水	塑颜新肌紧致精华水、水感透白光塑精华露
			婴儿爽身粉	婴儿清凉爽身粉、婴儿玉米爽身粉
		毛发	护发素	滋养水润润发精华素、植萃精华护发素
		口唇	润唇膏	修护型润唇膏、凝润滋养护唇膏
	美容修饰类	皮肤	粉饼	控油矿物质散粉
		皮肤	香体喷雾	爽身气雾
		毛发	发用啫喱	强力速型保湿性定型喷雾、臻研修护防热护发喷雾、强力定型啫喱水
	/	/	牙膏	草本水晶牙膏
特殊化妆品	特殊用途类	/	防晒	多效修护防晒霜SPF15P++、舒缓细肤倍护防晒乳SPF35P++、防晒乳SPF50PA+++

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[12]	Liyuan Zhang, Qiaoyuan Cheng, Cen Chen, Zehua Li, Liuqian Huang, Lvye Qi. Determination of 3 kinds of α-hydroxy acids and their esters in cosmetics by high performance liquid chromatography [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 102-106.
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[14]	Xu Han, Jiajia Wu, Na Wu, Yazhuo Shang. New emulsion system and its application in cosmetics （V） Janus emulsion [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 24-31.
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Applicability study of amplified ATP bioluminescence assay in microbial detection of cosmetics

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产品名称	批次	平板生长情况
产品名称	批次	黑曲霉	白色念珠菌	枯草芽胞杆菌	金黄色葡萄球菌	大肠埃希氏菌
洁面膏	Lot 1	—	/	/	/	/
	Lot 2	—	/	/	/	/
	Lot 3	—	/	/	/	/
氨基酸洁面泡泡	Lot 1	—	—	/	/	/
	Lot 2	—	—	/	/	/
	Lot 3	/	—	/	/	/
祛屑舒爽洗发乳液	Lot 1	—	—	—	—	—
	Lot 2	—	—	—	—	—
	Lot 3	—	—	—	—	—
爽身气雾	Lot 1	/	/	—	—
	Lot 2	/	/	—	/	/
	Lot 3	/	/	—	/	/