UHPLC-MS/MS法测定甘草类化妆品中光甘草定的研究

doi:10.3969/j.issn.1001-1803.2021.02.013

Abstract

Abstract:

An UHPLC-MS/MS method was developed for the determination of glabridin in cosmetic containing licorice. The glabridin in facial cream and toner samples was extracted with ethyl acetate and methanol, respectively. After optimizing the sample pretreatment and chromatography conditions, as well as mass spectrometry parameters, UHPLC-MS/MS analysis method was performed with MRM mode. The methodological parameters were investigated in detail. The results showed that the linear relationship is good and the detection limit is 0.006 mg/kg. The average recoveries of the two substrates ranged from 83.1% to 113% at low, medium and high mass concentrations. 31 Batches of samples labeled with licorice extracts were tested. Glabridin was detected in 13 batches with the content ranging from lower than LOQ to 944 mg/kg. The study can be used to evaluate the content of the active components of glycyrrhiza extract in cosmetics.

Key words: UHPLC-MS/MS, licorice, glabridin, cosmetic

CLC Number:

TQ658

DONG Ya-lei,QIAO Ya-sen,WANG Qi-lin,LI Li,HUANG Chuan-feng,WANG Hai-yan,SUN Lei. Determination of glabridin in cosmetic by UHPLC-MS/MS method[J].China Surfactant Detergent & Cosmetics, 2021, 51(2): 161-165.

Figures/Tables 5

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References 14

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序号	前处理方式	检测方法	保留时间/min	检出限	参考文献
1	70%甲醇提取两次,旋蒸浓缩二倍	HPLC-DAD	24	0.9 ng	[10]
2	50%甲醇直接提取	UPLC-DAD	10	0.032 1 ng	[11]
3	甲醇直接提取	HPLC-PDA	7.83	0.15 mg/L	[12]
4	甲醇提取,SPE净化	UPLC-Q-TOF MS	5.45	0.03 mg/kg	[13]
5	膏霜乳液和面膜,乙酸乙酯提取;液态水基样品,甲醇直接提取	UHPLC-MS/MS	5.12	0.006 mg/kg	本文

样品基质	本底值	加标值/（ng·mL^-1）	实测值/（ng·mL^-1）	回收率/%	RSD/%
膏霜乳液	0	4.92	4.09	83.1	4.3
		9.85	9.37	95.2	4.5
		19.7	16.0	91.6	0.9
液态水基质	0	4.92	4.93	100	1.8
		19.7	22.3	113	2.1
		49.2	55.1	112	1.0

序号	样品名称	是否特殊用途化妆品	标识成分	含量/（mg·kg^-1）
1	光果甘草焕颜霜	否	光果甘草根提取物	0.033 4
2	滋润倍护乳	否	光果甘草根提取物	0.037 3
3	美白嫩肤面膜	否	光果甘草提取物	0.585
4	雪肌精华水	否	光果甘草根提取物	1.67
5	美白嫩肤睡眠面膜	是	光果甘草根提取物	12.3
6	滋养霜	否	光果甘草根提取物	13.2
7	美白祛斑精华乳	是	光果甘草根提取物	26.0
8	修护眼唇霜	否	光果甘草根提取物	27.1
9	渗透乳	否	光果甘草根提取物	31.8
10	美白修复霜	是	光果甘草根提取物	520
11	雪肌精华液	否	光果甘草根提取物	944
12	健肤沐浴露	否	甘草根提取物	0.020 4
13	美白保湿柔肤水	是	甘草根提取物	检出,低于定量限

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Determination of glabridin in cosmetic by UHPLC-MS/MS method

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