重组人源化胶原蛋白基于体外水平的护肤功效研究

doi:10.3969/j.issn.2097-2806.2024.09.003

Abstract

Abstract:

Studying the skin care efficacy of recombinant humanized collagen based on in vitro level. The stability of the recombinant humanized collagen was first analyzed by treating at different temperatures, then its skincare efficacy based on in vitro level was evaluated by detecting the inhibition rate of elastase, the inhibition rate of collagenase, the protein content of type I collagen in human fibroblasts, the inhibition of reactive oxygen species （ROS） with human keratinocytes, and the effects of the recombinant humanized collagen on the expression of hyaluronic acid （HA）, filaggrin （FLG） and transglutaminase 1 （TGM1） in keratinocytes. The results showed that recombinant humanized collagen was able to maintain stability at temperatures below 70 ℃. With regard to its skincare efficacy, recombinant humanized collagen could inhibit elastase and collagenase activities and promote the increase of type I collagen content in human fibroblasts. It also showed good inhibition of ROS in keratinocytes in vitro and could increase the expression of HA, FLG, and TGM1 in keratinocytes. In short, the recombinant humanized collagen exhibited a favourable skin care effect in vitro level. This study proved that it has potential firming, anti-wrinkle, moisturizing, and repairing efficacy, and is a valuable cosmetic raw material.

Key words: recombinant humanized collagen, stability, human fibroblast cell, in vitro keratinocytes, skin care efficacy

CLC Number:

TQ658

Jian Wang, Yuhui Fan, Danfeng Li, Ningwen Cheng, Ling Li, Yufeng Yu. Skin care efficacy study of recombinant humanized collagen based on in vitro level[J].China Surfactant Detergent & Cosmetics, 2024, 54(9): 1030-1038.

Figures/Tables 10

Fig. 1

Fig. 2

Fig. 3

Tab. 1

Tab. 2

Fig. 4

Tab. 3

Fig. 5

Fig. 6

Fig. 7

References 23

[1]	Li Jie, Wang Mingchao, Qiao Yingyun, et al. Extraction and characterization of type Ⅰ collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering[J]. Process Biochemistry, 2018, 74: 156-163.
[2]	Fan Ting, Zhao Jianfeng, Chang Yejun, et al. Effect of recombinant humanized type Ⅲ collagen on expression of skin functional related genes[J]. China Surfactant Detergent & Cosmetics, 2022, 52 (12): 1326-1332.
[3]	Zhou Yuhan, Yuan Rongsui, Jiang Shoujun, et al. Biomedical application and development prospect of recombinant collagen[J]. Shanxi Chemical Industry, 2023, 43 (12): 29-31.
[4]	Chen Zhuoyue, Fan Daidi, Shang Lijun. Exploring the potential of the recombinant human collagens for biomedical and clinical applications: a short review[J]. Biom-edical Materials, 2021, 16 (1): 012001.
[5]	Ma Shuhua, Sun Yanan, Yang Weifeng, et al. Repair effect and mechanism of recombinant human collagen on laser induced damage of mice skin[J]. Acta Laser Biology Sinica, 2018, 27 (5): 399-406.
[6]	Yang Jianqiu, Gao Yihong, Zhu Hongliu. Therapeutic effect and mechanism of recombinant human type Ⅲ collagen functional gel on skin wound healing[J]. Shandong Medical Journal, 2021, 61 (34): 80-83.
[7]	Wang Jing, Qiu He, Xu Yang, et al. The biological effect of recombinant humanized collagen on damaged skin induced by UV-photoaging: an in vivo study[J]. Bioactive Materials, 2022, 11: 154-165. doi: 10.1016/j.bioactmat.2021.10.004 pmid: 34938920
[8]	Liu Hongye, Guo Shuping, Bai Li. Clinical observation of medical recombinant human collagen functional dressing for wound repair after carbon dioxide laser surgery[J]. Chinese Remedies & Clinics. 2015, 15 (10): 1458-1460.
[9]	Mao Zhengwei, Gao Changyou, Wang Dengyou, et al. Controlling biostability of collagen films for fibroblast cytocompatibility[J]. Journal of Bioactive and Compatible Polymers, 2004, 19 (5): 353-365.
[10]	Mu Changdao. A collagen facial mask and its preparation method: CN102138870A[P]. 2011-08-03.
[11]	Zhang Hui. Expression and purification of recombinant human collagen-like and its application in cosmetics[D]. Guangzhou: Jinan University, 2017.
[12]	He Xingxing, Zhao Yue, Lu Chaogang, et al. Study on the efficacy and evaluation of collagen, elastin and fibronectin in cosmetic[J]. Fine and Specialty Chemical, 2023, 31 (10): 39-43.
[13]	Wang Yulin. Research progress on the application of collagen in cosmetics[J]. The Science and Technology of Gelatin, 2012, 32 (1): 8-12.
[14]	Liang Meizhen. The application of protein in cosmetics[J]. Guangdong Chemical Industry, 2009, 36 (12): 104-105.
[15]	Lu Huanhuan, Zhou Xuye, Zhang Zhiwu. Study on effect of collagen type Ⅰ on tissue regeneration and anti-inflammation in zebrafish model[J]. Chinese Journal of Modern Applied Pharmacy, 2022, 39 (9): 1182-1186.
[16]	Nguyen T T, Happillon T, Feru J, et al. Raman comparison of skin dermis of different ages: focus on spectral markers of collagen hydration[J]. Journal of Raman Spectroscopy, 2013, 44 (9): 1230-1237.
[17]	Bodin J, Adrien A, Bodet P E, et al. Ulva intestinalis protein extracts promote in vitro collagen and hyaluronic acid production by human dermal fibroblasts[J]. Molecules, 2020, 25 (9): 2091.
[18]	Gu Yanpei, Han Jianxin, Jiang Chunpeng, et al. Biomarkers, oxidative stress and autophagy in skin aging[J]. Ageing Research Reviews, 2020, 59 (19): 101036.
[19]	Kanaki T, Makrantonaki E, Zouboulis C C. Biomarkers of skin aging[J]. Reviews in Endocrine & Metabolic Disorders, 2016, 17 (3): 433-442.
[20]	Tanha A, Rabiee M, Rostami A, et al. A green-based approach for noninvasive skin rejuvenation: potential application of hyaluronic acid[J]. Environmental Research, 2023, 234: 116467.
[21]	Li Wenhwa, Wong Hengkuan, Serrano J, et al. Topical stabilized retinol treatment induces the expression of HAS genes and HA production in human skin in vitro and in vivo[J]. Archives of Dermatological Research, 2017, 309 (4): 275-283. doi: 10.1007/s00403-017-1723-6 pmid: 28247017
[22]	Lai Q W S, Guo M S S, Wu K Q, et al. Edible bird’s nest, an asian health food supplement, possesses moisturizing effect by regulating expression of filaggrin in skin keratinocyte[J]. Frontiers in Pharmacology, 2021, 12: 685982.
[23]	Wolf C, Qian Y, Brooke M A, et al. ADAM17/EGFR axis promotes transglutaminase-dependent skin barrier formation through phosholipase Cγ1 and protein kinase C pathways[J]. Scientific Reports, 2016, 6: 39780.

	φ（RHC）/%
	100	80	60	40	20	10	1	0.1
Relative cell viability/%	2.50	3.30	20.92	47.35	81.88	95.50	98.20	101.40
SD	0.00	0.00	0.01	0.02	0.01	0.01	0.00	0.01

	φ（RHC）/%
	100	80	60	40	20	10	1	0.1
Relative cell viability/%	0.65	4.45	26.71	59.93	88.82	98.05	103.91	103.80
SD	0.00	0.00	0.00	0.01	0.01	0.01	0.00	0.01

Groups	ρ（Collagen type Ⅰ）/（pg/mL）	Collagen type Ⅰ increase rate/%	P-value
Negative Control	470.04±20.12	—	—
Positive Control	776.95±17.12	65.29	**
φ（RHC） =0.1%	772.24±27.92	64.29	**
φ（RHC） =1%	819.57±29.48	74.36	**
φ（RHC） =10%	876.83±22.00	86.54	**

Skin care efficacy study of recombinant humanized collagen based on in vitro level

RichHTML

PDF (PC)

Like

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 10

References 23

Related Articles 15

Metrics

Comments

Recommended 10

[1]	Meiling Shi, Juanbo Chen, Hao Li, Yuanqin Huang, Qiqing Zhang, Sa Zeng, Tao Meng. Study on the silicon dioxide encapsulation for the enhancement of vitamin C stability [J]. China Surfactant Detergent & Cosmetics, 2024, 54(9): 1015-1022.
[2]	Juyuan Zhang,Xiumei Tai,Huimin Liu,Xiaoyuan Ma,Xu Gao,Yi Luo. Effects of oil type on the stability and rheological properties of W/O Pickering emulsions [J]. China Surfactant Detergent & Cosmetics, 2024, 54(8): 879-886.
[3]	Siqi Yao,Yongli Yan,Suiwang Zhang,Yu Chen,Bingcheng He. Study on the non-aqueous foams stabilized by the modified nano-SiO₂particles [J]. China Surfactant Detergent & Cosmetics, 2024, 54(8): 903-910.
[4]	Fan Bai, Yongli Yan, Jiangbo Liu, Ayong Yan, Bingcheng He. Effects of oil on the formation and stability of aqueous foam [J]. China Surfactant Detergent & Cosmetics, 2024, 54(6): 630-639.
[5]	Xiaohong Pan, Ziqi Gao, Zhen Chen, Shuai Yin, Haiping Huang, Bin Hu. Discussion on the current situation of research and management on the stability of cosmetic products in China [J]. China Surfactant Detergent & Cosmetics, 2024, 54(2): 201-208.
[6]	Ziwei Zhang,Yongli Yan,Bingcheng He,Longlong Dou,Song Shi. Experimental study of non-aqueous foams stabilized with different surfactant aggregates [J]. China Surfactant Detergent & Cosmetics, 2024, 55(10): 1181-1190.
[7]	Baotong Jia,Kuan Chang. Preparation of phospholipid-based microemulsion and properties of loaded resveratrol [J]. China Surfactant Detergent & Cosmetics, 2024, 55(10): 1191-1200.
[8]	Chunfang Zhu,Junbiao Li,Zhenming Xie. Application of multiple emulsions in skin care cosmetics [J]. China Surfactant Detergent & Cosmetics, 2024, 55(10): 1243-1250.
[9]	Yaru Wang, Tingyuan Mo, Hongxia Lai, Yue Zhou, Jiaying Xie, Jianhua Tan. Analysis of the causes of skin irritation of niacinamide cosmetics based on patch test and stability test [J]. China Surfactant Detergent & Cosmetics, 2024, 54(1): 51-56.
[10]	Li Yitong, Sun Yanyan, Qiao Shaojun, Hou Lili, Li Manqin, Yu Yufeng. Effect of disinfectants on cell adhesion activity of recombinant type Ⅲ humanized collagen [J]. China Surfactant Detergent & Cosmetics, 2023, 53(7): 789-895.
[11]	Wang Huazheng, Zhang Liang, Kang Xin, Kang Wanli, Li Zhe, Yang Hongbin. Effect of CO₂ on physical properties of produced oil and water in Changqing and emulsion stabilization mechanism [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 617-624.
[12]	Zhen Enlong, Zhang Wen, Qian Zhen, Du Ruotong, Wang Yang. Construction and plugging performance evaluation of emulsified thermosetting resin system [J]. China Surfactant Detergent & Cosmetics, 2023, 53(6): 649-657.
[13]	Ding Zhengqing, Wu Yingyi, Wang Weiyun, Huang Xujuan, Cai Zhaosheng. Study on Pickering emulsion stabilized by hydroxyethyl cellulose/nanocellulose and its rheological properties [J]. China Surfactant Detergent & Cosmetics, 2023, 53(3): 245-252.
[14]	Yang Chao, Tong Zhiming, Wang Zhansheng, Chen Wu. Study on the influence mechanisms of polymers and solid particles on the stability of crude oil emulsion [J]. China Surfactant Detergent & Cosmetics, 2023, 53(10): 1156-1165.
[15]	Guo Fang. Influence of emulsifiers and thickener on formation of low-viscosity liquid crystal emulsions [J]. China Surfactant Detergent & Cosmetics, 2023, 53(1): 16-23.