乳酸促脂溢性脱发小鼠毛发生长研究

doi:10.3969/j.issn.2097-2806.2023.09.005

Abstract

Abstract:

The seborrheic alopecia model was established by removing the back hair of C57BL/6 mice and smearing with testosterone. The effects of lactic acid on hair growth, hair follicle growth cycle and growth factors in seborrheic alopecia mice were studied based on this model. Sixty C57BL/6 mice were randomly divided into normal group, model group, minoxidil tincture positive group, and groups of high-, medium- and low-dose lactic acid, which were smeared with corresponding drugs on the depilation area. After 24 days of continual administration, the time of skin color change, the hair growth in depilation area, the histological characteristics of hair follicles and the change of hair follicle number were recorded. The concentration of KGF, VEGF, and TGF-β1 in mouse skin was measured by corresponding Elisa kits. The results showed that, compared with the model group and normal group, lactic acid could significantly promote hair elongation in seborrheic alopecia mice, and increase the weight of mouse hair and the number of skin hair follicles in the drug-administration experimental area. Meanwhile, the concentrations of KGF and VEGF in mouse skin were up-regulated, and the concentration of TGF-β1 in mouse skin was reduced, both of which the differences were statistically significant （P<0.05）. The medium dose （10 g/L） of lactic acid solution had the best effect, which could accelerate the hair growth of mice, promote the transformation process of hair follicles in the growth period, and inhibit the symptoms of seborrheic alopecia. The curative effect of medium dose （10 g/L） of lactic acid solution was equivalent to that of minoxidil tincture （P>0.05）. It could be applied in daily care products to prevent alopecia and provide more choice for patients with seborrheic alopecia.

Key words: seborrheic alopecia, lactic acid, hair growth, keratinocyte growth factor, hair follicle cell

CLC Number:

TQ658

Luo Xiaojian, Hong Shixi, Luo Jing, Yang Si, Wang Ziyan, Xiong Yaokun. Study on the effect of lactic acid in promoting hair growth in seborrheic alopecia mice[J].China Surfactant Detergent & Cosmetics, 2023, 53(9): 1029-1034.

Figures/Tables 10

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References 21

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组别	t/d
正常组	7.2 ±0.92^*
米诺地尔酊组	7.4 ±0.87^*
脂溢性脱发模型组	8.25±1.17^▲
5 g/L乳酸溶液组	7.6 ±0.89
10 g/L乳酸溶液组	7.7 ±0.71
20 g/L乳酸溶液组	7.6 ±0.74

组别	第15天新生毛发长度/mm	第24天新生毛发长度/mm
正常组	5.40±0.23^*	10.47±0.19^**
米诺地尔酊组	5.01±0.95	10.26±0.82^**
脂溢性脱发模型组	4.71±0.75	8.75±1.07^▲
5 g/L乳酸溶液组	5.55±0.26^*	9.90±0.64^*
10 g/L乳酸溶液组	5.83±0.72^**	10.02±0.71^*
20 g/L乳酸溶液组	5.31±0.69^*	9.26±1.26

组别	小鼠新生毛发重量/mg
正常组	60.2±4.8^**
米诺地尔酊组	57.2±6.3^**
脂溢性脱发模型组	46.1±4.0 ^▲
5 g/L乳酸溶液组	44.5±3.4^▲
10 g/L乳酸溶液组	58.5±3.8^**
20 g/L乳酸溶液组	51.6±5.2^*

组别	平均毛囊数量/（个/视野）
正常组	27.80±4.12^**
米诺地尔酊组	25.33±2.37^*
脂溢性脱发模型组	19.27±3.74^▲
5 g/L乳酸溶液组	21.60±2.93
10 g/L乳酸溶液组	27.73±4.53^*
20 g/L乳酸溶液组	23.00±0.47

组别	ρ （KGF） /（×10^-6 g/L）	ρ （VEGF） /（×10^-6 g/L）	ρ （TGF-β1） /（×10^-6 g/L）
正常组	1.00±0.10^**	1.37±0.18^**	97±55^**
米诺地尔酊组	0.97±0.13^*	1.23±0.30^**	142±28^*
脂溢性脱发模型组	0.75±0.09^▲	0.77±0.11^▲	240±47^▲
5 g/L乳酸溶液组	0.81±0.18	0.87±0.10	173±30^*
10 g/L乳酸溶液组	0.96±0.08^**	1.09±0.20^**	180±17^*
20 g/L乳酸溶液组	0.81±0.21	0.84±0.11	175±22^*

Study on the effect of lactic acid in promoting hair growth in seborrheic alopecia mice

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