人参红景天乳液的制作及其抗氧化、美白作用研究

doi:10.3969/j.issn.2097-2806.2023.06.008

Abstract

Abstract:

The best mixing ratio of Rhodiola rosea extract and Panax ginseng extract was determined, and this mixture was used as a raw material to prepare a lotion. The process was optimized by orthogonal experiment. The antioxidant and whitening effects of the lotion were studied by DPPH, ABTS, PTIO free radical scavenging tests, measurement of total flavonoid content, mouse B16 melanoma cell test, tyrosinase and melanin inhibition tests, and melanocyte oxidative stress-related protein expression test. The results showed that, when the ratio of Rhodiola rosea extract to Panax ginseng extract in the lotion was 5∶3, the optimal formula was obtained. The optimized conditions were emulsification temperature of 80 ℃, emulsification time of 20 min, and emulsification speed of 4 000 r/min. The total flavonoid content of the lotion thus obtained was 1.84 mg/mL.The scavenging rates for DPPH, ABTS and PTIO free radicals were 40.17%, 48.27% and 38.22%, respectively. The inhibition rates on tyrosinase and melanin were 40.49% and 35.64%, respectively. The expression of oxidative stress-related proteins was high in the middle- and high-dose groups. Therefore, the lotion containing Rhodiola rosea and Panax ginseng had good antioxidant activity and whitening efficacy.

Key words: Rhodiola rosea, Panax ginseng, lotion, anti-oxidant, whitening

CLC Number:

TQ658

Han Yudi, Jin Liying, Sun Xihan, Lin Changqing, Cui Chengbi. Preparation of a lotion containing Rhodiola rosea and Panax ginseng and its antioxidant and whitening effects[J].China Surfactant Detergent & Cosmetics, 2023, 53(6): 665-672.

Figures/Tables 16

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References 15

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组分	化学名称	含量/%
A	去离子水	至100
	1，3-丁二醇	7.00
	甘油	1.00
	1，2-己二醇	2.00
	无水甜菜碱	0.50
	尿囊素	1.00
	D-泛醇	2.00
	黄原胶	0.05
	卡波姆-940	0.10
B	去离子水	5.00
	PEG-60氢化蓖麻油	0.50
	乙基己基甘油	0.15
C	红景天提取物	3~5
	人参提取物	2~3
	氨丁三醇	0.10~0.18
D	芳香油	0.05

反应组	V_PTIO溶液/mL	V_样品溶液/mL	V_PBS/mL
A_对照	1.8		0.2
A_样品	1.8	0.2
A_空白		0.2	1.8

组别	pH=6.8 PBS	样品	L-酪氨酸溶液（质量分数0.1%）	酪氨酸酶溶液（配制有磷酸盐缓冲溶液，质量浓度为200 μg/mL）
阴性对照组（A_A）	0.75		0.40	0.25
阳性对照组（A_B）	1.00		0.40
受试组（A_E）	0.35	0.40	0.40	0.25
空白组（A_F）	0.60	0.40	0.40

序号	人参提取物/%	红景天提取物/%	DPPH自由基清除率/%
0	0	0	0
1	2	3	60.46
2	2	4	61.93
3	2	5	68.32
4	2.5	3	66.07
5	2.5	4	64.35
6	2.5	5	65.84
7	3	3	70.97*
8	3	4	72.72*
9	3	5	74.10*

因素	A 乳化温度/℃	B 乳化时间/min	C 乳化速度/（r/min）
1	60	10	4 000
2	70	20	5 000
3	80	30	6 000

Preparation of a lotion containing Rhodiola rosea and Panax ginseng and its antioxidant and whitening effects

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试验号	A 乳化温度	B 乳化时间	C 乳化速度	空列	总分
1	1	1	1	2	2.37
2	1	2	3	1	2.89
3	1	3	2	3	3.76
4	2	1	3	3	5.34
5	2	2	2	2	7.06
6	2	3	1	1	4.53
7	3	1	2	1	7.41
8	3	2	1	3	7.74
9	3	3	3	2	6.46
K₁	9.02	15.12	14.64	14.83
K₂	16.93	17.69	18.23	15.89
K₃	21.61	14.75	14.69	16.84
k₁	3.01	5.04	4.88	4.94
k₂	5.64	5.90	6.08	5.30
k₃	7.20	4.92	4.90	5.61
R	4.19	0.98	1.20	0.67

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因素	平方和	自由度	F值	P值	显著性
A	26.998	2	15.551	0.004	**
B	1.709	2	0.168	0.849
C	2.825	2	0.288	0.759