逆境胁迫处理后山楂叶总黄酮含量变化及对心肌缺血性大鼠分子机制的研究

doi:10.3969/j.issn.1001-1803.2023.04.009

Abstract

Abstract:

The total flavonoids in different varieties of hawthorn leaves were determined and the molecular mechanism of the effects on myocardial ischemia in rats was discussed. The seedlings of different varieties of hawthorn trees with good growth and uniform size were selected, and the total flavonoids of hawthorn leaves were extracted by organic solvent extraction. The content was determined by HPLC and ultraviolet spectrophotometry. The antioxidant capacity was determined by ultraviolet spectrophotometry. The total flavonoids were then applied to myocardial ischemic model rats, and the molecular mechanism was determined by electrocardiography, biochemical index detection and Real-Time PCR technology. The results showed that, the content of total flavonoids in the hawthorn leaves of “Dajinxing” and the antioxidant capacity thereof were significantly higher than other varieties; the total flavonoids in hawthorn leaves could significantly improve the ST segment status, biochemical indexes and hemodynamic parameters of ECG in acute myocardial ischemic rats; total flavonoids of hawthorn leaves could significantly affect the expression of CaSR, c-fos and Cyt-C genes in myocardial ischemic rats. In conclusion, among the four varieties of hawthorn leaves, the content of total flavonoids and antioxidant capacity of “Dajinxing” hawthorn leaves were significantly higher than those of other groups, which could significantly improve the state of myocardial ischemic rats, providing a theoretical basis for the selection of high-quality crude drug sources in future.

Key words: total flavones of hawthorn leaves, antioxidant capacity, acute myocardial ischemia, molecular mechanism

CLC Number:

TQ658

Ren Jiejie. Change in total flavonoid content of hawthorn leaves after adverse stress treatment and the molecular mechanism on myocardial ischemia in rats[J].China Surfactant Detergent & Cosmetics, 2023, 53(4): 430-436.

Figures/Tables 9

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References 26

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组别	脯氨酸含量/（μg/mg FW）	w（可溶性糖）/%
野生型	0.482±0.015	0.138±0.017
“大红”	0.793±0.018	0.205±0.013
“大金星”	0.874±0.019	0.271±0.016
“大旺”	0.737±0.023	0.196±0.011
“敞口”	0.812±0.021	0.235±0.015

组别	处理前ST段最大值（MV）	处理后ST段最大值（MV）
组别	处理前ST段最大值（MV）	1 min	3 min	5 min	10 min	15 min
空白对照组	0.090±0.032	0.090±0.032	0.091±0.005	0.089±0.007	0.090±0.008	0.091±0.006
模型对照组	0.087±0.027	0.134±0.024	0.112±0.021	0.081±0.018	0.079±0.019	0.087±0.025
阳性药物处理组	0.088±0.015	0.112±0.017	0.094±0.018	0.092±0.011	0.088±0.024	0.089±0.021
山楂叶总黄酮处理组	0.089±0.022	0.113±0.018	0.089±0.020	0.090±0.023	0.089±0.019	0.088±0.017

组别	测定项目	处理前	处理后
组别	测定项目	处理前	1 min	3 min	5 min	10 min
空白对照组	HR/（beat/min）	332.21±21.34	10.83±0.25	7.54±0.58	8.32±0.57	10.08±0.63
	LVEDP/kPa	4.34±0.38	5.03±0.39	2.62±0.31	3.15±0.06	3.51±0.07
	+dp/dt_max/（kPa/s）	446.25±13.65	16.70±1.24	10.47±0.72	8.70±0.90	6.40±0.42
	-dp/dt_max/（kPa/s）	435.52±28.36	12.95±0.53	11.30±0.62	4.65±0.36	3.47±0.05
模型对照组	HR/（beat/min）	321.70±12.80	82.81±8.94	78.52±1.36	49.69±2.64	15.53±0.67
	LVEDP/kPa	3.96±0.74	6.03±0.32	15.41±0.84	8.72±0.46	5.35±0.42
	+dp/dt_max/（kPa/s）	478.29±43.47	97.54±8.46	79.67±3.18	37.57±1.15	18.21±0.36
	-dp/dt_max/（kPa/s）	449.45±28.85	100.62±7.59	86.78±4.54	44.34±2.63	11.68±0.53
阳性药物处理组	HR/（beat/min）	328.56±20.32	45.67±4.54	34.25±13.65	23.73±1.52	9.73±0.84
	LVEDP/kPa	4.12±0.35	5.34±0.41	7.14±0.21	5.08±0.06	4.13±0.16
	+dp/dt_max/（kPa/s）	455.52±15.58	35.15±0.26	16.56±0.47	8.62±0.56	6.34±0.15
	-dp/dt_max/（kPa/s）	441.57±19.49	43.41±1.34	25.74±0.56	12.08±0.41	8.78±0.52
山楂叶总黄酮处理组	HR/（beat/min）	329.03±16.43	61.55±1.86	35.78±2.68	24.82±0.75	10.21±0.73
	LVEDP/kPa	4.17±0.53	5.41±0.37	7.92±0.81	5.63±0.46	4.62±0.26
	+dp/dt_max/（kPa/s）	448.14±25.46	36.52±0.54	17.54±0.62	9.08±0.80	6.74±0.41
	-dp/dt_max/（kPa/s）	440.43±21.32	48.31±3.72	25.71±0.96	12.71±0.84	9.05±0.63

组别	T-AOC/（U/mL）
空白对照组	14.05±0.29^**
模型对照组	7.34±0.52
阳性药物处理组	11.79±0.75^**
山楂叶总黄酮处理组	11.84±0.37^**

组别	Na⁺-K⁺-ATP含量/（U/mg prot）	Ca²⁺-Mg²⁺-ATP含量/（U/mg prot）
空白对照组	4.57±0.35	6.38±0.42
模型对照组	2.82±0.41	3.52±0.56
阳性药物处理组	3.84±0.23	4.78±0.41
山楂叶总黄酮处理组	3.89±0.25	4.93±0.56

Change in total flavonoid content of hawthorn leaves after adverse stress treatment and the molecular mechanism on myocardial ischemia in rats

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组别	n	CK-MB/（UL）	ρ（cTnI）/（ng/mL）	ρ（MyoG）/（ng/mL）
空白对照组	20	5.56±0.41^**	1.63±0.14^**	82.68±12.35^**
模型对照组	20	114.22±24.35	8.62±0.58	345.35±2.97
阳性药物处理组	20	63.67±3.63^*	3.89±0.41^*	158.14±12.74^*
山楂叶总黄酮处理组	20	61.89±2.74^*	3.67±0.47^*	160.17±10.56^*

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