同位素稀释-气相色谱-质谱法测定化妆品中大麻二酚和四氢大麻酚

doi:10.3969/j.issn.2097-2806.2023.11.014

Abstract

Abstract:

A method for the determination of cannabidiol （CBD） and tetrahydrocannabinol （THC） in cosmetics was established by using isotope dilution-gas chromatography-mass spectrometry （GC-MS）. The samples were ultrasonically extracted with acetonitrile, then the sample solution was prepared after centrifugation, and the analytes were separated on HP-5MS column （30 m×250 μm×0.25 μm）, detected by full-scan and selected-ion monitoring, quantified by isotope （Tetrahydrocannabinol-D3，THC-D3） internal standard method. The results show that the standard curves have good linear relationship in the range of 0.05-1.0 mg/L for CBD and THC, with the linear correlation coefficients greater than 0.995. The detection limits of the method （S/N=3） are 0.05, 0.20 mg/kg, respectively, and the spiked recoveries in three levels are between 92.0%-111.1% with the RSDs of 0.40%-9.9%. The method is simple, rapid, high sensitivity and suitable for the determination of cannabidiol and tetrahydrocannabinol in cosmetics.

Key words: cannabidiol, tetrahydrocannabinol, gas chromatography-mass spectrometry（GC-MS）, cosmetics, isotope internal standard method

CLC Number:

TQ658

Li Changyu, Hu Dan, Liao Shangfu, Lu Yan, Yang Ming. Determination of cannabidiol and tetrahydrocannabinol in cosmetics by isotope dilution-gas chromatography-mass spectrometry[J].China Surfactant Detergent & Cosmetics, 2023, 53(11): 1340-1344.

Figures/Tables 6

Tab. 1

Fig. 1

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References 15

[1]	Chen Qijun, Zhu Bo, Chen Weisheng, et al. Simultaneous determination of tetrahydrocannabinol and tetrahydro-cannabinolic acid in urine by liquid chromatography coupled with tandem mass spectrometry after dispersive solid-phase extraction[J]. Chinese Journal of Health Laboratory Technology, 2016, 26 (11) : 1535-1538.
[2]	Aisikaer Aierken, Sun Liyang, Xie Hui, et al. Detecting contents of cannabis and its metabolites in human blood and urine by gas chromatography-mass spectrometry (GC-MS) method[J]. Journal of Xinjiang Medical University, 2016, 39 (8) : 1020-1025.
[3]	Paul R, Williams R, Hodson V, et al. Detection of cannabinoids in hair after cosmetic application of hemp oil[J]. Scientific Reports, 2019 (9) : 2582-2587.
[4]	Wang Zhanliang, Zhang Jianli, Zhang Yinong. Simultaneous determination of the cannabinol, cannabidiol and Δ9-tetrahydrocannabinol in athletic nutrition by gas chromatography-mass spectrometry[J]. Chinese Journal of Sports Medicine, 2015, 34 (4) : 398-403.
[5]	Sun Dongmei, Song Lun, Wang Haiyan, et al. Establishment of detection methods for five cannabinoids in hemp cosmetics based on HPLC[J]. Analytical Sciences, 2021, 37: 1821-1824. doi: 10.2116/analsci.21P158 pmid: 34148924
[6]	Ren Yanming, Xie Runfang, Wang Rui, et al. Rapid determination of eight cannabinoids in cannabis plants by ultra performance liquid chromatography-tandem mass spectrometry[J]. Journal of Yunnan University: Natural Sciences Edition, 2021, 43 (6) : 1212-1219.
[7]	Li Zhining, Wei Yue, Li Feifei, et al. Determination of CBD, THC and CBN in hemp from different habitats by technique-isotopic internal standard method based on GC-MS[J]. Chinese Journal of Pharmaceutical Analysis, 2021, 41 (11) : 1894-1903.
[8]	Qian Zhenhua, Qiao Hongwei, Hua Zhendong. Determination of 8 synthetic cannabinoids by gas chromatography-mass spectrometry[J]. Chinese Journal of Forensic Medicine, 2015, 30 (1) : 1-4.
[9]	Yan Zhiwei, Guan Jiangling, Ding Bin. GC/MS/SIM Method for quantitative detection of main components in interplanting cannabis plants[J]. Journal of Criminal Investigation Police University of China, 2021 (5) : 115-121.
[10]	Li Fajin, Zhang Yunbo, Gong Deming. Determination of cannabidiol in cosmetics by high performance liquid chromatography[J]. Detergent & Cosmetics, 2021, 44 (10) : 46-52.
[11]	Chen Niannian, Zhou Ying, Han Li, et al. Determination of tetrahydrocannabinol, cannabidiol and cannabinol in cosmetics by ultra performance liquid chromatography-triple quadrupole mass spectrometry[J]. Detergent & Cosmetics, 2022, 45 (5) : 18-23.
[12]	Li Shuo, Li Li, Wang Haiyan, et al. Determination of cannabidiol in cosmetics by ultra-performance liquid chromatography-tandem mass spectrometry[J]. China Surfactant Detergent & Cosmetics, 2021, 51 (2) : 166-170.
[13]	Sun Weilai, Zheng Xiaoyu, Zhao Yanbiao, et al. Simultaneous determination of Δ9-THC and Δ9-THCA-A in cannabis plant by UPLC-MS[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2018, 54 (6) : 644-649.
[14]	Xu Xiuming. LC-MS/MS determination of 6 synthetic cannabinoids in plant sample[J]. Physical Testing and Chemical Analysis (Part B: Chemical Analysis), 2017, 53 (11) : 1291-1295.
[15]	Tang Qingqiang, Wu Chundeng, Cao Xiaogang, et al. Determination of nine cannabinoids in incense smoke by gas chromatography-mass spectrometry combined with solid phase extraction-isotope dilution[J]. Journal of Instrumental Analysis, 2022, 41 (5) : 761-767.

序号	化合物	保留时间/min	选择离子（m/z）
1	CBD	12.415	231^*	246	314
2	THC	12.811	231^*	299	271
3	THC-D3	12.799	302^*	317	234

化合物	基质溶液中目标物质量浓度/（mg/L）			纯溶剂中目标物质量浓度/（mg/L）	基质效应/%
化合物	水剂	乳液	膏霜	纯溶剂中目标物质量浓度/（mg/L）	水剂	乳液	膏霜
CBD	0.364 9	0.560 7	0.505 1	0.3	21.63	86.90	68.37
THC	0.301 5	0.501 1	0.475 8	0.3	0.50	67.03	58.60

序号	化合物	线性方程	相关系数R²	检出限/（mg/kg）	定量限/（mg/kg）
1	CBD	y=3.648x+0.451 1	0.999 8	0.05	0.15
2	THC	y=0.768x+0.135 8	0.999 6	0.20	0.60

化合物	添加量/（mg/kg）	水剂		膏霜		乳液
化合物	添加量/（mg/kg）	回收率/%	RSD/%	回收率/%	RSD/%	回收率/%	RSD/%
CBD	2.0	102.2	1.5	110.8	6.2	92.0	4.4
	6.0	99.1	0.4	98.9	1.8	93.8	2.4
	16.0	101.1	1.1	98.2	0.4	99.5	1.2
THC	2.0	103.5	6.5	111.1	2.3	101.3	9.9
	6.0	100.6	1.2	108.9	0.9	102.7	2.3
	16.0	102.7	1.3	105.6	1.6	103.6	1.4

Determination of cannabidiol and tetrahydrocannabinol in cosmetics by isotope dilution-gas chromatography-mass spectrometry

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