阿魏酸纳米醇质体的制备、表征及体外经皮吸收研究

doi:10.3969/j.issn.2097-2806.2023.11.007

Abstract

Abstract:

The transdermal delivery and antioxidant of ferulic acid nanoethosomes prepared by microfluidization were studied. Firstly, the effects of edge-activators （Tween-80, and sucrose palmitate） and lecithin on the encapsulation efficiency of ferulic acid nanoethosomes were investigated by the response surface method. When lecithin, sucrose palmitate and Tween-80 are optimized at the ratio of 0.68%:0.51%:0.25% （w/%）, the ferulic acid nanoethosomes demonstrate better characteristics of small particle size of （104.5±0.7） nm, uniform particle size distribution with PDI of 0.05±0.01, high encapsulation efficiency of 88.8%±2.6%, and high storage stability for 60 days at 25 ℃. Secondly, the antioxidant ability and the skin penetration property of ferulic acid and its nanoethosomes were evaluated by DPPH free radical scavenging tests and in vitro transdermal assays using Franz diffusion cells, respectively. The IC₅₀ values of ferulic acid and its nanoethosomes are 8.7 and 11.7 μg/mL, respectively, indicating that the ferulic acid nanoethosomes exhibite enhanced antioxidant activity. It is found that the nanoethosomes have better skin permeability and their skin adsorption amount and accumulated permeation amount of the nanoethosomes are （18.2±3.5） μg/cm² and （25.3±3.2） μg/cm², which are 1.2 times and 1.4 times higher than that of the ferulic acid propylene glycol solution, respectively. The prepared ferulic acid nanoethosomes have the advantages of uniform particle size, high encapsulation efficiency and storage stability, enhanced antioxidant ability and skin permeability, and can be extended to the preparation of other phenolic acid nanoethosomes and applied as transdermal carriers for antioxidant, anti-aging, and whitening cosmetics.

Key words: ferulic acid, nanoethosomes, microfluidization, antioxidant, skin penetration

CLC Number:

TQ658

Deng Mengjie, Yi Guobin, Lv Ran, Liu Yafeng, Ye Dawei, Chen Jiazhi. Ferulic acid nanoethosomes: preparation, characterization, and performance of skin penetration in vitro[J].China Surfactant Detergent & Cosmetics, 2023, 53(11): 1285-1292.

Figures/Tables 8

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References 18

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水平	因素
水平	A（卵磷脂含量/%）	B（蔗糖棕榈酸酯含量/%）	C（吐温-80含量/%）
-1	0.1	0.4	0.1
0	0.5	0.5	0.25
1	0.9	0.6	0.4

方差来源	平方和	自由度	均方	F值	P值
模型	4 634.75	9	514.97	80.03	<0.000 1
A	2 489.36	1	2 489.36	386.87	<0.000 1
B	4.40	1	4.40	0.68	0.44
C	0.11	1	0.11	0.02	0.90
AB	0.18	1	0.18	0.03	0.87
AC	37.52	1	37.52	5.83	0.05
BC	48.72	1	48.72	7.57	0.03
A²	1 706.26	1	1 706.26	265.17	<0.000 1
B²	19.52	1	19.52	3.03	0.13
C²	220.89	1	220.89	34.33	0.00
残差	45.04	7	6.43
失拟	38.01	3	12.67	7.21	0.04
纯误差	7.03	4	1.76
总和	4 679.79	16
R²=0.990 4，R²_Adj=0.978 0

Ferulic acid nanoethosomes: preparation, characterization, and performance of skin penetration in vitro

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序号	因素			Y（EE/%）
序号	A（卵磷脂含量/%）	B（蔗糖棕榈酸酯含量/%）	C（吐温-80含量/%）	Y（EE/%）
1		0.4	0.25	40.95
2	0.1	0.5	0.4	45.21
3	0.1	0.5	0.1	37.15
4	0.1	0.6	0.25	45.44
5	0.5	0.6	0.1	71.95
6	0.5	0.4	0.4	71.59
7	0.5	0.5	0.25	84.95
8	0.5	0.5	0.25	85.52
9	0.5	0.5	0.25	82.34
10	0.5	0.5	0.25	84.95
11	0.5	0.5	0.25	85.52
12	0.5	0.4	0.1	80.03
13	0.5	0.6	0.4	77.47
14	0.9	0.4	0.25	79.73
15	0.9	0.5	0.4	71.29
16	0.9	0.6	0.25	83.37
17	0.9	0.5	0.1	80.03

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