Abstract:

The active bacteria in 20 commercially available probiotic toothpastes were detected by conventional methods, and high-throughput sequencing of the V3 and V4 regions of 16S rDNA. The amplicon analysis was used for α Diversity and Microbial Community Structure analysis. Finally, based on the specific primers, the PCR method was used to detect Lactobacillus paracasei in 20 toothpastes. 1） The results show that the total bacterial count of 20 probiotic toothpastes is <10 CFU/g, and the total lactic acid bacteria is <10 CFU/g. 2） Through analyzing the amplicon PCR sequencing results, lactobacillus other than Lactobacillus casei are added to the toothpaste, 18 toothpastes add Lactobacillus fermentans, Lactobacillus acidophilus and Lactobacillus delbrueckii subspecies, 20 toothpastes add Lactobacillus plantarum, and 19 toothpastes add Lactobacillus reuteri. The sequencing diversity analysis show that the abundance of prokaryotic microorganisms and the diversity of colonies in the toothpaste are high. 3） The results of PCR and sequencing of toothpaste DNA extracts show that Lactobacillus paracasei is added to 20 toothpastes according to the label.

Key words: probiotic toothpaste, amplicon sequencing, Lactobacillus paracasei