甘草次酸对金黄地鼠皮脂腺斑影响的实验研究

doi:10.3969/j.issn.1001-1803.2022.06.007

Abstract

Abstract:

This experiment preliminarily confirmed that glycyrtinic acid at the mass concentration of 50-100 μg/mL could significantly inhibit the secretion of triglyceride by 3T3-L1 cells （P<0.01） in a mass concentration-dependent manner. 3T3-L1 cells, as preadipocytes, continue to increase adipocyte proliferation and triglyceride accumulation under exogenous stimulation. They differentiate into mature adipocytes and form adipose tissue. Glycyrrhetinic acid can inhibit the proliferation of preadipocytes and reduce the accumulation of triglycerides, indicating that glycyrrhetinic acid has the effect of inhibiting lipid synthesis and metabolism at the cellular level. Further, through the golden hamsters model, it is confirmed that glycyrrhetic acid at the set mass concentration significantly reduces the area of bilateral sebaceous gland on the abdomen of golden hamsters, reduces the thickness and number of sebaceous glands, inhibites the growth of sebaceous glands and thus reduces the abnormal excessive secretion of sebaceous glands. At the mass concentration of 100 μg/mL, glycyrrhetinic acid significantly regulates the expression of SrebP-1C （P<0.001）, TG （P<0.01）, K6 （P<0.01）, K16 （P<0.01） and IL-6 （P<0.05） in sebaceous glands. It inhibits the SREBP protein activity in sebaceous gland tissue, inhibits sebum synthesis and secretion of triglyceride composition content, and further reduces the cutin formation and keratin K6, K16 expression in cells, which play a role in regulating sebaceous gland growth and oil secretion. Abnormal sebum secretion from sebaceous glands can induce skin health problems such as acne, skin barrier dysfunction and specific dermatitis. Through the above experimental studies, it has been preliminarily confirmed that the single use of glycyrrhetinic acid can effectively inhibit the growth of sebaceous gland spots in golden hamsters and reduce sebum secretion and inflammatory reaction. These results suggest that glycyrrhetinic acid may play a role in regulating oily skin, inhibiting acne, improving skin barrier function and specific dermatitis, and can be used as an active ingredient in skin surgery drugs and functional daily cosmetics that relieve acne and skin inflammation.

Key words: glycyrrhetinic acid, sebaceous gland, golden hamsters, anti-inflammatory

CLC Number:

TQ658

He Yifei,Lu Wangwang,Ma Shijing,Liu Guanting,Wang Mengping,Du Zhiyun. Experimental study on the effect of glycyrrhetinic acid on sebaceous gland of golden hamsters[J].China Surfactant Detergent & Cosmetics, 2022, 52(6): 620-625.

Figures/Tables 6

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References 22

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实验组	甘草次酸质量浓度/（μg/mL）	3T3-L1细胞活性/%	毒性分级
空白对照组		102.10±1.05	0
甘草次酸组	25	99.13±1.36	1
	50	98.58±1.76	1
	100	97.69±1.07	1
	125	71.99±0.55	3

实验组	甘草次酸质量浓度/（μg/mL）	TG含量/（μmol/L）
空白对照组		24.63±3.51
模型组		49.63±4.38^###
甘草次酸组	25	46.53±4.09
	50	33.51±3.80^**
	100	26.07±3.12^***

组别	给药前		给药后
组别	左侧/mm²	右侧/mm²	左侧/mm²	右侧/mm²
空白对照组	37.57±3.11	35.88±1.91	58.92±1.90 ^a	57.50±3.82 ^a
基质组	39.02±4.48	36.24±3.66	59.71±1.17 ^b	61.76±2.81 ^b
甘草次酸低剂量（50 μg/mL）组	36.83±2.95	35.96±4.80	47.20±2.80 ^{**, ##}	49.80±1.79 ^{**, ##}
甘草次酸高剂量（100 μg/mL）组	38.05±2.36	37.48±5.07	45.71±1.94 ^{***, ###}	44.16±2.32 ^{***, ###}

实验组	皮脂腺厚度/mm	皮脂腺数量/个
空白对照组	0.872±0.080	42.00±5.48
基质组	0.838±0.068	41.00±3.56
甘草次酸低剂量（50 μg/mL）组	0.561±0.084^**,##	31.25±2.75^**,##
甘草次酸高剂量（100 μg/mL）组	0.432±0.093^***,###	23.00±2.58^***,###

实验组	SREBP-1C/（pg/mL）	TG/（μmol/mL）	K6/（pg/mL）	K16/（pg/mL）	IL-6/（pg/mL）
空白对照组	876.02±25.81	1.88±0.24	24.82±2.39	56.01±4.21	27.31±2.71
基质组	849.38±32.27	1.83±0.37	26.36±1.18	59.87±2.08	30.81±4.01
甘草次酸低剂量（50 μg/mL）组	615.77±19.63^**,##	1.32±0.13^*,#	20.27±2.09	43.29±4.18^**,##	24.27±2.20
甘草次酸高剂量（100 μg/mL）组	528.03±35.1^***,###	1.06±0.22^**,##	18.06±0.95^**,##	42.05±1.92^**,##	20.15±2.42^*,#

Experimental study on the effect of glycyrrhetinic acid on sebaceous gland of golden hamsters

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