重组人源化Ⅲ型胶原蛋白对皮肤功能性相关基因 表达的影响

doi:10.3969/j.issn.1001-1803.2022.12.010

Abstract

Abstract:

At present, the extraction of collagen is mainly from animal skin tissues, such as pigs, cattle, fish, and so on, which has certain virus hidden trouble. In order to obtain safer and better collagen, scholars have used gene recombination technology to produce recombinant human collagen. Type Ⅲ collagen is the main collagen in human skin, so the use of recombinant type Ⅲ collagen is of great significance for the study of skin properties. The recombinant humanized type Ⅲ collagen is obtained by optimizing recombinant expression of a human collagen original gene sequence that is highly consistent with a human natural collagen amino acid sequence. In order to explore the effect of recombinant humanized type Ⅲ collagen on alleviating skin wrinkles when applied to cosmetics, its anti-wrinkle effect was verified at the skin functional gene level by testing on human skin fibroblasts and keratinocytes. The results show that 5 mg/mL RHC Ⅲ can significantly promote the expression of collagen synthesis-related genes Collagen Ⅰ, Collagen Ⅲ and Smad3 in human skin fibroblasts. Meanwhile, under UVA stimulation, the expression of collagen degradation related genes MMP-1, MMP-3, c-jun and c-fos can be significantly inhibited. Acting on keratinocytes can significantly promote the expression of skin barrier and moisturizing-related genes LOR, TGM1, IVL, OCLN1, DSG1, FLG, Caspase14 and K1.To sum up, the sample recombinant type Ⅲ collagen has anti-wrinkle effect at the mass concentration of 5 mg/mL.

Key words: recombinant humanized type Ⅲ collagen, collagen synthesis and degradation, barrier repair and moisturizing, anti-wrinkle

CLC Number:

TQ658

Fan Ting,Zhao Jianfeng,Chang Yejun,Ji Le. Effect of recombinant humanized type Ⅲ collagen on expression of skin functional related genes[J].China Surfactant Detergent & Cosmetics, 2022, 52(12): 1326-1332.

Figures/Tables 9

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Effect of recombinant humanized type Ⅲ collagen on expression of skin functional related genes

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