7种植物精油对糠秕马拉色菌抑制效果比较及樟树精油抑菌作用研究

doi:10.3969/j.issn.1001-1803.2022.11.008

Abstract

Abstract:

By comparing the inhibitory activities of seven essential oils against Malassezia furfur, the most effective ones were selected and further analyzed. The essential oils with the best antibacterial effects were determined by K-B disk diffusion method and broth microdilution method. In addition, the volatile components of the essential oils with the best antibacterial effects were identified by gas chromatography-mass spectrometry （GC-MS）, and further correlation analysis was performed between the main chemical components and the activity against Malassezia furfur. Then, the morphological changes of Malassezia furfur were observed by scanning electron microscopy （SEM） and transmission electron microscopy （TEM）. Finally, the effect of Malassezia furfur on cell apoptosis was detected by flow cytometry. The results showed that, Cinnamomum camphora essential oil was more active against Malassezia furfur than the essential oils from Osmanthus fragrans, Citrus limon, Lavandula angustifolia, Rosmarinus officinalis, Melaleuca alternifolia, and Citrus bergamia. A total of 14 chemical components were identified by GC-MS, and the inhibitory activity against Malassezia furfur was derived from 3-carene, terpinene-4-ol, α-terpinene, terpinolene; SEM showed that Cinnamomum camphora essential oil caused mild damage to the cells of Malassezia furfur, and the local cell membrane was depressed; TEM showed that Cinnamomum camphora essential oil caused the plasmolysis of Malassezia furfur, and the intracellular matrix was lightened. Cinnamomum camphora essential oil significantly increased the apoptosis of Malassezia furfur. In conclusion, Cinnamomum camphora essential oil has a significant inhibitory effect against Malassezia furfur by destroying the cell wall and cell membrane.

Key words: Cinnamomum camphora essential oil, Malassezia furfur, SEM, TEM, apoptosis

CLC Number:

TQ658

He Yuan,Li Ziqiang,Sun Weijie,Ma Shumei,Liu Li. Inhibitory effects of seven plant essential oils against Malassezia furfur and the antimicrobial mechanism of Cinnamomum camphora essential oil[J].China Surfactant Detergent & Cosmetics, 2022, 52(11): 1202-1207.

Figures/Tables 7

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References 20

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精油	抑菌圈直径/mm	最低抑菌浓度MIC/（μL/mL）
樟树（Cinnamomum camphora）	15.58±1.26	8
桂花（Osmanthus fragrans）	12.79±0.57	32
柠檬（Citrus limon）	15.51±0.89	16
薰衣草（Lavandula angustifolia）	11.04±1.12	32
迷迭香（Rosmarinus officinalis）	10.94±0.89	32
茶树（Melaleuca alternifolia）	10.03±0.03	32
佛手柑（Citrus bergamia）	9.73±0.15	64

化合物序号	化合物	分子式	保留时间/min	相对含量/%
1	莰烯（Camphene）	C₁₀H₁₆	4.002	1.7
2	3-蒈烯（3-Carene）	C₁₀H₁₆	4.101	6.56
3	桧烯（Sabinene）	C₁₀H₁₆	4.532	11.43
6	柠烯（Cinene）	C₁₀H₁₆	5.004	2.26
7	α-松油烯（α-Terpinene）	C₁₀H₁₆	5.136	3.98
8	1，8-桉叶油素（1，8-Eucalyptol）	C₁₀H₁₈O	5.217	48.02
9	γ-松油烯（γ-Terpinene）	C₁₀H₁₆	5.471	0.88
10	异松油烯（Terpinolene）	C₁₀H₁₆	5.846	3.11
11	芳樟醇（Linalool）	C₁₀H₁₈O	6.723	0.95
12	松油烯-4-醇（Terpinen-4-ol）	C₁₀H₁₈O	6.851	4.58
13	α-松油醇（α-Terpineol）	C₁₀H₁₈O	6.995	10.15
14	石竹烯（Caryophyllene）	C₁₅H₂₄	9.304	1.75
15	α-律草烯（α-Humulene）	C₁₅H₂₄	9.658	0.76
16	香树烯（Alloaromadendrene）	C₁₅H₂₄	9.995	1.09

单体	抑菌圈直径/mm
3-蒈烯（3-Carene）	24.18±0.65
松油烯-4-醇（Terpinen-4-ol）	41.54±1.79
α-松油烯（α-Terpinene）	16.29±0.23
异松油烯（Terpinolene）	37.18±1.91

组别	凋亡百分数/%
对照组	1.82±0.24
1 MIC	3.94±0.91^*
2 MIC	5.31±0.82^**

Inhibitory effects of seven plant essential oils against Malassezia furfur and the antimicrobial mechanism of Cinnamomum camphora essential oil

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