虫草素纳米乳液制备及对皮肤光老化修复机制研究

doi:10.3969/j.issn.1001-1803.2022.10.006

Abstract

Abstract:

Cordycepin was used as an effective component to prepare nanoemulsion. The formulation process, preliminary quality evaluation and skin photoaging repair mechanism of cordycepin nanoemulsion were studied. By drawing the pseudo ternary phase diagram, the formula of the nanoemulsion was determined. The particle size, viscosity, and stability of the nanoemulsion were investigated. The activity of superoxide dismutase （SOD）, glutathione （GSH）, catalase （CAT） and the content of malondialdehyde （MDA） in the skin of New Zealand rabbits were measured. The results show that the nanoemulsion is stable when the Km of surfactant and co-surfactant is 2∶1. The average diameter of cordycepin nanoemulsion is 374 nm and the viscosity is （9.33±0.14） mPa·s. It has good centrifugal stability, dilution stability, long-term stability and temperature stability. Cordycepin can be maintained at （12.006±0.025） mg/g for 1-8 months with the RSD within 1%, and it begins to decrease in the ninth month. The activities of SOD, CAT and GSH in group 4 using cordycepin nanoemulsion are slightly lower than those in the control group, significantly higher than those in groups 1, 2 and 3 （P<0.01）. The MDA in the control group and group 4 are significantly lower than those in groups 1, 2 and 3 （P<0.01）. Cordycepin nanoemulsion has good stability, viscosity and particle size, and can effectively alleviate skin aging caused by ultraviolet radiation. This study aims to lay a theoretical foundation and provide direction for the further development of the function of cordycepin nanoemulsion.

Key words: cordycepin, nanoemulsion, high performance liquid chromatography, anti-aging, stability

CLC Number:

TQ658

Zhang Hucheng,Yang Guowei,Yang Jun,Fan Haitao,Luo Shuai,Liu Linying. Preparation of cordycepin nanoemulsion and its repair mechanism on the photoaged skin[J].China Surfactant Detergent & Cosmetics, 2022, 52(10): 1072-1080.

Figures/Tables 11

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References 26

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组相	原料名称	质量分数/%
A相	鲸蜡醇	2.0
	聚二甲基硅氧烷（硅油）	3.0
	鲨烷	4.0
	山梨坦硬脂酸酯	3.0
B相	卡波姆	0.12
	黄原胶	0.05
	甘油	3.0
	透明质酸钠	0.03
	1,3-丁二醇	1.00
	EDTA-二钠	0.03
	维生素C乙基醚	1.5
	聚季铵盐-51	0.2
C相	库拉索芦荟	2.5
	神经酰胺-3	1.2
	二裂酵母发酵产物溶胞物	1.00
	苯氧乙醇	0.70
D相	虫草素	1.20

储存时间/月	外观	平均粒径/nm
1	乳白均匀、未见分层及破乳	375.88±1.23
6	乳白均匀、未见分层及破乳	381.65±2.15
12	乳白均匀、未见分层及破乳	392.39±1.84

	平均粒径/nm
	4 ℃	25 ℃	40 ℃
0 h	370.4±2.280	370.4±3.758	370.4±6.507
24 h	371.7±3.420	374.5±4.540	469.4±5.347
30 d	378.5±5.704	375.9±1.230	1 015.3±7.880
一个月后粒径增加值	8.1	34.5	644.9

进样次数	保留时间/min	保留时间平均值/min	RSD/%	峰面积	峰面积平均值	RSD/%
1	14.151	14.113	0.35	1 977.245	1 982.611	0.32
2	14.057			1 989.765
3	14.131			1 980.824

次数	虫草素质量浓度/（µg/mL）	标准品虫草素加样质量浓度/（µg/mL）	测得总虫草素质量浓度/（µg/mL）	回收率/%
1	12.00	5.00	16.96±0.08	99.765
2	12.00	10.00	21.93±0.04	99.682
3	12.00	15.00	26.95±0.03	99.815

Preparation of cordycepin nanoemulsion and its repair mechanism on the photoaged skin

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