采用DPRA替代方法评价3种香料的皮肤致敏性

doi:10.3969/j.issn.1001-1803.2021.05.011

Abstract

Abstract:

The sensitization of lyral, chloroatranol and atranol was evaluated by direct peptide reaction test （DPRA） . The DPRA method was established in our lab. The polypeptide elimination rate of lyral, chloroatranol, atranol and six other compounds with known sensitization were measured by HPLC, and the skin sensitization was then predicted. The feasibility of DPRA method was determined by comparing its consistency with LLNA results, and the sensitization of the three perfumes was finally determined. The sensitization results of the six known compounds as evaluated by DPRA and LLNA were consistent, and the DPRA predictions of the three perfumes were positive. The DPRA method was simple and accurate, and could be used as an alternative method for skin sensitization evaluation.

Key words: direct peptide reaction test （DPRA）, skin sensitization, perfume, lyral, chloroatranol, atranol

CLC Number:

TQ658

YANG Ting-ting,YI Lu-yao,WANG Yi,LI Jie,JI Wei-jia,ZHANG Hong. Evaluation of skin sensitization of three perfumes by using DPRA alternative method[J].China Surfactant Detergent & Cosmetics, 2021, 51(5): 438-442.

Figures/Tables 5

Tab. 1

Tab. 2

Fig. 1

Tab. 3

Fig. 2

References 14

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半胱氨酸和赖氨酸消耗百分比的均值（x）/%	反应等级	DPRA预测结果
0 ≤x≤6.38	无或轻微反应	阴性
6.38<x≤22.62	弱反应	阳性
22.62<x≤42.47	中度反应	阳性
42.47<x≤100	强反应	阳性

受试物	半胱氨酸多肽消耗率/%	赖氨酸多肽消耗率/%	多肽平均消耗率/%	反应等级	DPRA 预测结果	LLNA 参考结果
甲醛	26.34±0.76	100.00±0.00	63.2	强反应	阳性	强致敏物
6-甲基香豆素	0.49±0.08	3.49±0.53	2.0	无	阴性	非致敏物
对苯二胺	100.00±0.00	5.02±0.35	52.5	强反应	阳性	强致敏物
对氨基苯甲酸	0	0	0	无	阴性	非致敏物
异丙醇	0	0	0	无	阴性	非致敏物
肉桂醛	100.00±0.00	46.11±4.05	73.1	强反应	阳性	强致敏物
新铃兰醛	100.00±0.00	5.90±1.14	53.0	强反应	阳性	/
氯化苔黑醛	62.29±13.54	86.85±5.19	74.6	强反应	阳性	/
苔黑醛	3.09±0.43	21.69±1.49	12.4	弱反应	阳性	/

Evaluation of skin sensitization of three perfumes by using DPRA alternative method

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t/min	φ（流动相A）/%	φ（流动相B）/%	流速/（mL·min^-1）
0	90	10	1.00
12	48	52	1.00
13	10	90	1.00
15	10	90	1.00
16	90	10	1.00
20	90	10	1.00