复方精油对小鼠B16细胞内黑色素生成影响

doi:10.3969/j.issn.1001-1803.2021.03.008

Abstract

Abstract:

In order to investigate the inhibitory effect of a blend essential oil on tyrosinase activity and melanin content, the composition of this blend essential oil was analyzed by GC-MS. Taking mouse melanoma cells （B16） with high melanin expression induced by melanocyte-stimulating hormone （α-MSH） as the model, the effects of the blend essential oil on melanocyte vitality were determined by trypan blue staining and CKK8 method. Enzyme-linked immunosorbent assay （ELISA） and dopa oxidation method were used to measure melanin content and tyrosinase activity in cells. The results showed that the blend essential oil contained a large number of compounds such as pinene （26.19%） , linalool （12.98%） , santalol （9.39%） , linalyl acetate （9.16%） , etc.; the blend essential oil had no toxicity to B16 cells when the mass concentration was less than 0.1 g/L, and the cell proliferation rate was higher than 90%; the blend essential oil at different mass concentrations could significantly inhibit the tyrosinase activity and melanin content in B16 cells induced by α-MSH. When the mass concentration of blend essential oil was 0.1 g/L,it had the best inhibitory effect on melanin production, and the inhibitory rates on tyrosinase activity and melanin synthesis were 51.27% and 36.03%, respectively. In conclusion, the blend plant essential oil could significantly inhibit melanin synthesis and inhibit the tyrosinase activity in B16 cells.

Key words: compound essential oil, melanin content, tyrosinase inhibition, B16 melanoma cell

CLC Number:

TQ658

WANG Ying,TANG Yin,WU Ya-ni. Effects of blend essential oil on melanogenesis in B16 cells of mice[J].China Surfactant Detergent & Cosmetics, 2021, 51(3): 214-219.

Figures/Tables 6

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Tab. 1

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References 19

[1]	Zhang Wei, Gao Jing, Zhang Min, et al. In vitro biological effects of seven whitening compounds on skin melanocytes[J]. Journal of Pharmaceutical Practice, 2011,29(1) ：24-26, 40.
[2]	Scur M C, Pinto F G, Pandini J A, et al. Antimicrobial andantioxidant activity of essential oil and different plant extracts of psidium cattleianum sabine[J]. Brazilian Journal of Biology, 2016,76(1) ：101-108.
[3]	Jimbow K, Park J S, Kato F, et al. A ssembly, target-singnling and intracellular transport of tyrosinase gene family proteins in the initial stage of melanosome biogenesis[J]. Pigment Cell Res, 2000,13:222-229. pmid: 10952389
[4]	Kushuimoto T, Basrur V, Valencia J, et al. A model for melanosome biogenesis based on the purification and analysis of early melanosomes[J]. PANS, 2001,98(19) ：10698-10703.
[5]	Sun Bei, Li Xiao. Research progress on skin whitening preparations that affect melanin deposition and its mechanism of action[J]. Chinese Journal of Aesthetic Medicine, 2015,24(22) ：82-85.
[6]	Li Y M, Jia M, Li H, et al. Cnidium monnieri: a review of traditional uses, phytochemical and ethnopharmacological properties[J]. American Journal of Chinese Medicine, 2015,43:835-877.
[7]	Qiu Ling. Study on the mechanism of tyrosinase action by natural plant active substances[D]. Xiamen: Xiamen University, 2009.
[8]	Enos T A, Eri M, Irawan W K, et al. Inhibitory components from the buds of clove (Syzygium aromaticum) on melanin formation in B16 melanoma cells[J]. Fitoterapia, 2011 (82) ：198-202.
[9]	Chou S T, Chang W L, Chang C T, et al. Cinnamomum cassia essential oil inhibits α-MSH-Induced melanin production and oxidative stress in murine B16 melanoma cells[J]. International Journal of Molecular Sciences, 2013,14:186-201.
[10]	Ma Lin, Luo Jiaquan, Li Dan, et al. Comparison between real-time unlabeled cell analysis system and trypan blue staining method to analyze the cytotoxicity of bisphenol A on mouse testicular interstitial cells[J]. Practical Medicine and Clinical, 2018,21(9) ：978-980.
[11]	Yang Hongqiu. Resveratrol induces apoptosis of melanoma A375 cells by regulating PI3K/AKT/mTOR signaling pathway[D]. Sichuan: Southwest Medical University, 2018.
[12]	Yuan Bing, Li Ranran, Han Mingyong. Experimental study of malignant melanoma regulating lung microenvironment and promoting lung metastasis[J]. Lebensmittel Wissenschaft and Technologie, 2016,17:13-17.
[13]	Maack A, Pegard A. Populus nigra (Salicaceae) absolute rich in phenolic acids, phenylpropano ds and flavonoids as a new potent tyrosinase inhibitor[J]. Fitoterapia, 2016,111:95-101. pmid: 27091790
[14]	Ellouze I, Abderrabba M, Sabaou N, et al. Season’s variation impact on Citrus aurantium leaves essential oil: chemical composition and biological activities[J]. Journal of Food Science, 2012,77(9) ：173-180.
[15]	Peng H Y, Lin C C, Wang H Y, et al. The melanogenesis alteration effects of Achillea millefolium L. essential oil and linalyl acetate: Involvement of oxidative stress and the JNK and ERK signaling pathways in melanoma cells[J]. PloS One, 2014 (9) ：95-186.
[16]	Misra, Dey. TLC-bioautographic evaluation of in vitro anti-tyrosinase and anti-cholinesterase potentials of sandalwood oil[J]. Nat Prod Commun, 2013,8:253-256. pmid: 23513742
[17]	Ritaro M, Hiroyuki U, Masayoshi S. Tyrosinase inhibitory activity of Citrus essential oils[J]. Journal of Agricultural and Food Chemistry, 2006,54:2309-2313. doi: 10.1021/jf051682i pmid: 16536612
[18]	Kang J, Jin W, Wang J, et al. Antibacterial and anti-biofilm activities of peppermint essential oil against Staphylococcus aureus[J]. Food Science and Technology, 2019,101:639-645.
[19]	Azmi N, Hashim P, Hashim D M, et al. Anti-elastase, anti-tyrosinase and matrix metalloproteinase-1 inhibitory activity of earthworm extracts as potential new anti-aging agent[J]. Asian Pacific Journal of Tropical Biomedicine, 2014,4(1) ：S348-S352.

序号	化学成分	保留时间/min	相对含量/%
1	松油烯（Terpinene）	13.22	0.01
2	α-蒎烯（α-Pinene）	14.41	26.19
3	莰烯（Camphene）	14.81	1.31
4	月桂烯（Myrcene）	15.97	6.9
5	邻伞花烃（o-Cymene）	17.04	0.11
6	柠檬烯（Limonene）	17.53	8.81
7	桉叶醇（Eucalyptol）	17.64	1.63
8	马鞭草烯醇（Verbenol）	19.43	0.29
9	芳樟醇（Linalool）	19. 85	12.98
10	顺式马鞭草烯醇（cis-Verbenol）	21.32	0.98
11	松油烯-4-醇（Terpinen-4-ol）	22.69	4.36
12	萜品醇（Terpineol）	23.07	1.07
13	香茅醇（Citronellol）	23.86	1.01
14	乙酸芳樟酯（Linalyl acetate）	24.71	9.16
15	乙酸冰片酯（Bornyl acetate）	25.90	0.66
16	乙酸桃金娘烯酯（Myrtenyl acetate）	26.20	0.07
17	乙酸松油酯（Terpinyl acetate）	27.72	0.06
18	胡椒稀（Copaene）	28.63	0.14
19	β-倍半水芹烯（β-sesquiphellandrene）	29.12	3.19
20	榄香烯（Elemene）	29.47	0.02
21	石竹烯（Caryophyllene）	30.24	4.97
22	蛇麻烯（Humulene）	31.21	1.58
23	红没药醇（Bisabolol）	31.45	0.01
24	大根香叶烯（Germacrene D）	31.92	1.15
25	依兰油烯（Muurolene）	32.32	2.02
26	甜没药烯（Bisabolene）	32.45	0.15
27	橙花椒醇（Nerolidol）	33.79	0.15
28	石竹烯氧化物（Caryophyllene oxide）	34.73	0.72
29	桃金娘烯醇（Myrtenol）	36.69	0.84
30	檀香醇（Santalol）	38.34	9.39
31	水芹烯（Phellandrene）	42.36	0.04
32	金合欢烯（Farnesene）	43.61	0.02

Effects of blend essential oil on melanogenesis in B16 cells of mice

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