Abstract:

A method for the determination of octyl hydroxamic acid in cosmetics by high performance liquid chromatography （HPLC） was established. ZORBAX Eclipse Plus C₁₈ column （4.6 mm×250 mm, 5 μm） was used. The mobile phase was water and acetonitrile, the flow rate was 1.0 mL/min, the column temperature was 25 ℃, the detection wavelength was 220 nm and the injection volume was 10 μL. The results show that the peak area has a good linear relationship with the mass concentration of octanoyl hydroxamic acid in the range of 10-200 mg/L, and the correlation coefficient is 0.999 9 （n=5）. The limit of detection is 5 mg/kg. The recoveries of octyl hydroxamic acid at different spiked levels are 96.28%-101.23% with the relative standard deviations （RSD） less than 2.43%. This method is simple operation, high precision, high recovery and good reproducibility, and can be used to detect octyl hydroxamic acid in cosmetics.

Key words: cosmetics, high performance liquid chromatography, octyl hydroxamic acid