发酵法制备沙棘籽粕总黄酮的初步纯化及抗衰老活性评价

doi:10.3969/j.issn.1001-1803.2019.10.006

Abstract

Abstract:

The fermentation extract of sea-buckthorn seed meal was purified by macroporous adsorption resin. The technological conditions in the purification process were studied, as well as the anti-aging activity of the extract. The effects of separation and purification of three macroporous adsorption resins were compared. The optimum separation conditions with X-5 macroporous resin were investigated. The effect of the extract on cell growth was evaluated by MTT method. The anti-aging activity of the extract was evaluated by DPPH method, the inhibition of the activities of intracellular reactive oxygen species and MMP-1, and the promotion of collagen synthesis. The results show that the optimum process conditions: adsorption time of 2 h, adsorption temperature of 15 ℃, eluent volume fraction of 60%, elution time of 2.5 h, and elution temperature of 45 ℃. Under such conditions, the adsorption amount could reach 2.33 mg/g, and the resolution rate could reach 94.75%. The MTT experiment showed that it had no toxic side effects on fibroblasts; the mass concentration when the DPPH radical scavenging rate reaches 50%（IC₅₀）was 1.06 mg/L; the level of reactive oxygen species decreased significantly; the MMP-1activity was decreased; the collagen content was increased. The fermentation broth of sea-buckthorn seed meal showed good anti-aging activity.

Key words: additive of cosmetics, sea-buckthorn seed meal, flavonoid, fermentation, anti-aging

CLC Number:

TQ658

YOU Shi-quan,WANG Chang-tao,ZHANG Jia-chan,LI Meng,ZHAO Dan,AN Quan. Preliminary purification and anti-aging activity evaluation of total flavonoids prepared from sea-buckthorn seed meal by fermentation[J].China Surfactant Detergent & Cosmetics, 2019, 49(10): 661-667.

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References 19

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θ/℃	15	25	35	45	55	–
t/h	0.5	1	1.5	2	2.5	3
φ（洗脱剂）/%	40	50	60	70	80	–

水平	A 料液比/（g∶mL）	B 接菌量/%	C pH
1	1∶60	4	4
2	1∶70	6	5
3	1∶80	8	6

水平	因素				黄酮提取量/（mg·g^-1）
水平	A	空列	B	C	黄酮提取量/（mg·g^-1）
1	1	1	1	1	8.343
2	1	2	2	2	9.376
3	1	3	3	3	8.254
4	2	1	2	3	9.986
5	2	2	3	1	10.976
6	2	3	1	2	11.865
7	3	1	3	2	8.876
8	3	2	1	3	11.976
9	3	3	2	1	11.232
K₁	25.973	29.205	31.184	29.551
K₂	32.827	31.328	29.594	32.117
K₃	32.084	30.351	30.106	29.216
k₁	8.658	9.735	10.395	9.850
k₂	10.942	10.443	9.865	10.706
k₃	10.695	10.117	10.035	9.739
R	2.285	0.708	0.359	0.855

Preliminary purification and anti-aging activity evaluation of total flavonoids prepared from sea-buckthorn seed meal by fermentation

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