基于皮肤模型与THP-1细胞共培养建立皮肤致敏体外检测方法的初步研究

doi:10.13218/j.cnki.csdc.2018.05.009

Abstract

Abstract: An in vitro skin sensitization test based on skin model and THP-1 cell co-culture was established,and used to evaluate the sensitizing potency of petrochemicals.Skin model was treated with different concentrations of samples for 15 min,then removing the samples and was incubated for another 6 h.Skin tissue activity was measured by MTT assay,while cell viability and cell surface markers (CD54,CD86) expression levels of THP-1 cells were detected by flow cytometry.The skin sensitization potential of test substances was determined by statistically analyzing the above parameters and reaching a dose-response relationship.The results of SLS,cinnamaldehyde and citral show that this co-culture method can correctly distinguish irritants and sensitizers.The results of 4 unknown lubricant additives show that HVI60,Cuvan826 and ATM PAN are predicted to be non-sensitizers,while Irgamet30 is predicted to be a sensitizer,which at higher concentration can decrease the skin tissue activity and THP-1 cell viability,and specifically increase the expression of surface markers.

Key words: skin sensitization, skin model, THP-1 cells, co-culture

CLC Number:

CHAN Jian, KE Yi-hui, TAN Wei-jun. A preliminary study on in vitro skin sensitization test based on skin model and THP-1 cell co-culture[J].China Surfactant Detergent & Cosmetics, 2018, 48(5): 288-292.

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A preliminary study on in vitro skin sensitization test based on skin model and THP-1 cell co-culture

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