Abstract: Via the use of Mimedisc^® as a scaffold,the normal human dermal fibroblasts were first seeding into the scaffold to form a dermal equivalent after 3 weeks;and then seeding normal human epidermal keratinocytes on it,followed by immersion and air-liquid inter-phase culture stage,to finally form a 3D sponge matrix skin model which has a very similar structure of normal human skin (NHS).The results of Masson staining,immuno-histochemical (IHC) and immuno-fluorescence (IF) staining show that this skin model displays a very similar structure and key protein expression of NHS.Meanwhile,this model was used as a tool to investigate the effect of Himalaya cedar extract on Dermal-Epidermal Junction (DEJ) in vitro.Results show that the Himalayan cedar extract can significantly increase the thickness of the epidermis,thus increase the barrier capacity of the skin to resist external environment.Further,the Himalaya cedar extract can promote the expression of Laminin 5 and Collagen VII of DEJ.The expression of the Laminin 5 and Collagen VII increases 20% and 47% respectively.So the Himalaya cedar extract displays the potential to be applied in development of anti-aging cosmetics.

Key words: cosmetic raw materials, cedar, 3D skin model, Dermal-Epidermal Junction, anti-aging