Abstract: Water extraction and alcohol precipitation were used to obtain sapindosides,and total yield of the sapindosides was taken as the evaluation index.Effect of three factors,mass fraction of the primary paste obtained,volume fraction of ethanol and alcohol precipitation temperature were examined with orthogonally designed experiments.Results showed that mass fraction of the primary paste is the major factor,while volume fraction of ethanol and alcohol precipitation temperature are the minor factors.The recommended conditions for the extraction process is 25% for mass fraction of the primary paste,70% for volume fraction of ethanol and 30 ℃ for alcohol precipitation temperature.Under these conditions,3.659 1 g of sapindosides can be obtained from 100 g of peel of sapindus tree fruit.

Key words: sapindoside, water extraction and alcohol precipitation method, optimization, orthogonal test