黄芩与丹参配伍面膜剂的成型工艺及抗菌活性研究

doi:10.3969/j.issn.2097-2806.2025.03.009

Abstract

Abstract:

Acne is a common skin disease, and the occurrence of acne is closely related to Propionibacterium acnes. Research shows that Scutellaria baicalensis Georgi and Salvia miltiorrhiza Bge. have good antibacterial activities, especially to Propionibacterium acnes. The facial mask combined S. baicalensis Georgi with S. miltiorrhiza Bge. was prepared, and its forming process was optimized through response surface test. A rapid analysis technology for active ingredients was established using high performance liquid chromatography （HPLC）, and the experiments were held to investigate the antibacterial activities of Propionibacterium acnes and Staphylococcus aureus in vitro. The results show that three main factors affecting the forming process are glycerol, 1, 3-butanediol and hydroxyethyl cellulose identified by single factor test. Box-Behnken test and response surface method are used to optimize the variation parameters of the three significant factors, and its optimal process conditions. The twelve indicative activities in the facial mask are identified by HPLC, and the rapid quality analysis method and its chromatographic conditions are established. Also, the bacteriostatic test in vitro of the facial mask confirms that it shows certain bacteriostatic effects on Propionibacterium acens and Staphylococcus aureus, and can exert effectively and continuously within ten hours. When it is combined with positive drug DP-300, it shows better synergistic or additive bacteriostatic effects, which are considered as the mild bacteriostatic effects due to facial mask.

Key words: Scutellaria baicalensis Georgi, Salvia miltiorrhiza Bge., facial mask, acne, antibacterial activity

CLC Number:

TQ658

Weiqiang Song, Yali Chen, Hongyan Yang, Fan Zhang. Research on the forming process and antibacterial activity of the facial mask combined Scutellaria baicalensis Georgi with Salvia miltiorrhiza Bge.[J].China Surfactant Detergent & Cosmetics, 2025, 55(3): 333-340.

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References 11

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水平	丙三醇/g（A）	1， 3-丁二醇/g（B）	羟乙基纤维素/g（C）
-1	5	3	0.1
0	7	4	0.3
+1	9	5	0.5

实验组	因素			保湿率/%
实验组	A	B	C	保湿率/%
1	7	4	0.3	91.88
2	7	3	0.5	92.33
3	9	5	0.3	92.42
4	5	3	0.3	87.60
5	7	4	0.3	92.49
6	5	4	0.5	88.73
7	7	3	0.1	84.17
8	9	3	0.3	86.81
9	7	5	0.1	92.20
10	7	5	0.5	90.66
11	5	5	0.3	88.60
12	9	4	0.5	89.96
13	7	4	0.3	92.20
14	7	4	0.3	91.80
15	9	4	0.1	88.47
16	5	4	0.1	86.64
17	7	4	0.3	92.40

方差来源	平方和	自由度	均方	F值	P值
模型	105.85	9	11.76	53.43	<0.000 1**
A	4.68	1	4.68	21.27	0.002 4**
B	21.13	1	21.13	95.97	<0.000 1**
C	13.00	1	13.00	59.08	0.000 4**
AB	5.38	1	5.38	24.45	0.001 7
AC	0.090 0	1	0.090 0	0.408 8	0.542 9
BC	23.52	1	23.52	106.86	<0.000 1**
A²	23.05	1	23.05	104.69	<0.000 1**
B²	3.80	1	3.80	17.24	0.004 3**
C²	7.84	1	7.84	35.61	0.000 6**
残差	1.54	7	0.220 1
失拟项	1.17	3	0.388 3	4.13	0.102 0
纯误差	0.375 9	4	0.094 0
总误差 R²=0.985 7	107.39 R²_Adj=0.967 2	16

名称	线性范围/ （μg/mL）	标准曲线	r
野黄芩苷	20~400	Y=5.351 1X-59.169	0.999 5
迷迭香酸	15~300	Y=4.033 7X+5.887 5	0.999 3
黄芩苷	20~750	Y=9.402 7X+33.658	0.999 7
丹酚酸B	20~750	Y=2.968 3X-143.91	0.999 3
汉黄芩苷	20~334	Y=13.673X+47.944	0.999 0
去甲汉黄芩素	0.4~266	Y=24.572X+67.281	0.999 1
汉黄芩素	2~33	Y=29.206X-15.399	0.999 1
千层纸素A	2~324	Y=18.381X+51.334	0.999 2
二氢丹参酮Ι	0.6~216	Y=16.847X+19.592	0.999 3
隐丹参酮	2.4~334	Y=13.199X+15.488	0.999 0
丹参酮Ι	2~300	Y=4.727X+23.198	0.999 1
丹参酮ⅡA	2~350	Y=20.668X+43.002	0.999 1

成分	含量/（μg/g）						平均含量/（μg/g）
成分	S1	S2	S3	S4	S5	S6	平均含量/（μg/g）
野黄芩苷	223.12	212.17	221.08	208.09	238.03	220.02	220.42±10.37
迷迭香酸	70.08	68.98	72.12	68.89	70.34	62.86	68.87±3.17
黄芩苷	2 678.91	2 590.09	2 580.88	2 789.04	2 671.09	2 780.61	2 681.77±89.42
丹酚酸B	1 578.85	1 528.67	1 656.04	1 520.02	1 678.09	1 570.92	1 587.57±65.21
汉黄芩苷	619.09	612.83	640.98	587.03	607.89	610.09	612.99±17.49
去甲汉黄芩素	7.03	6.88	6.16	6.89	7.02	6.93	6.81±1.32
汉黄芩素	41.07	41.37	38.88	43.86	43	40	41.36±1.84
千层纸素A	10.12	11.02	9.93	10.36	11.01	10.45	10.48±0.45
二氢丹参酮Ι	4.08	3.59	3.82	4.02	3.79	3.86	3.86±0.17
隐丹参酮	23.34	24.09	22.67	22.08	23.87	23.48	23.25±0.75
丹参酮Ι	10.08	10.85	11.09	9.92	10.08	10.52	10.42±0.47
丹参酮ⅡA	6.02	5.48	6.02	5.82	5.81	5.96	5.85±0.20

Research on the forming process and antibacterial activity of the facial mask combined Scutellaria baicalensis Georgi with Salvia miltiorrhiza Bge.

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药物	痤疮丙酸杆菌		金黄色葡萄球菌
药物	MIC/（mg/mL）	MBC/（mg/mL）	MIC/（mg/mL）	MBC/（mg/mL）
DP-300	2.5	2.5	1.25	1.25
面膜制剂	250	250	500	500

菌株	面膜剂/（mg/mL）		DP-300/（mg/mL）		FIC
菌株	MIC（单）	MIC（联）	MIC（单）	MIC（联）	FIC
痤疮丙酸杆菌	250	31.25	2.5	0.63	0.375
金黄色葡萄球菌	500	250	1.25	0.16	0.625

试药	供试细菌	回归方程和相关系数	IC₁₀/（μg/mL）	IC₅₀/（μg/mL）	IC₉₀/（μg/mL）
DP-300	痤疮丙酸杆菌	+16.28，r=0.991 1	8.554	140.6	2 311
DP-300	金黄色葡萄球菌	+290.8，r=0.992 1	78.40	430.2	2 361
面膜剂	痤疮丙酸杆菌	+260.1，r=0.968 8	15 230	99 100	644 800
面膜剂	金黄色葡萄球菌	+2.232，r=0.988 1	13 310	73 060	400 900

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