拟微绿球藻发酵肽的抗衰老作用研究

doi:10.3969/j.issn.2097-2806.2024.08.006

Abstract

Abstract:

The extraction and composition analysis of peptides （FNPs） from fermentation Nannochloropsis sp., as well as their anti-aging effects on Drosophila melanogaster in vivo were investigated. Compared with the non-fermentation group, the number of peptide in FNPs increases by about 100, and the oxidation sites in the peptides also increase. The maximum absorption intensity of FNPs is observed at 258 nm with a dose-dependent relationship. Compared with the control group, 5 μg/mL FNPs extends the mean life span of the the natural aging Drosophila melanogaster for 13 days. Additionally, for 70-90 days old, the deterioration of oxidative stress indicators such as MDA, SOD, and CAT caused by age are significantly restored and increased to the level of positive group by FNPs at 0.5 and 5 μg/mL. These results indicate that FNPs have potential as a promising nutritional component for alleviating human health problems induced by aging. It can also be used as a new antioxidant ingredient for functional food development or cosmeceutical applications from a resource rich and easily obtainable raw material Nannochloropsis sp..

Key words: bioactive peptide, anti-aging, fermented Nannochloropsis sp., Drosophila melanogaster

CLC Number:

TQ658

Ting Yang,Huawen Li,Xun Xu,Honghui Guo,Tangbin Zou,Enqin Xia. The anti-aging potential of peptides from fermented Nannochloropsis sp.[J].China Surfactant Detergent & Cosmetics, 2024, 54(8): 921-929.

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References 29

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	All	Score>60	Score>120	Score>180	Top score	Unique（Groups）	Unique（Peptides）	Oxidant sites
NPs	196	148	36	6	252.21	180	130	12
FNPs	292	229	78	11	268.93	262	184	16

Peptide sequence	Proteins	Length	Score	Intensity	M-IDs
EMIESGVIVASDLSK	T1RHE4	15	170	4.11×10⁷	30
DSIESLVNYMK	T1RJ27	11	140	3.31×10⁷	49
MLEPTVPGIVER	T1RIQ6	12	137	2.73×10⁸	43
STMLEPLIVGDEHYQIAQDVK	T1RHE4	21	133	4.43×10⁷	32
IFDLMDAVDSYIPTPVR	T1RHG5	17	124	1.39×10⁷	39
TVLIMELINNVAK	T1RHE4	13	118	1.88×10⁷	34
VALSALTMAEYFR	T1RHE4	13	115	1.16×10⁸	35
VATVPIGPDMLGR	T1RIQ6	13	104	8.76×10⁷	44
TIAMNPTEGLAR	T1RHE4	12	101	1.00×10⁸	33
SMNPDAGLIFSYYK	A0A4V8H021	14	87	1.37×10⁷	17
MPLFDIK	T1RIS7	7	84	1.55×10⁷	45
VALVYGQMNEPPGAR	T1RHE4	15	82	2.97×10⁷	36
FMEEAPTFVK	A0A4V8H021	10	79	1.93×10⁷	16
DASLFEMPTGGTAK	T1RIT2	14	67	1.05×10⁷	46
VPSAVGYQPTLATEMGMLQER	T1RHE4	21	64	1.01×10⁷	37; 38
ELIVGAMAK	T1RJX6	9	63	2.39×10⁶	53

	Linear range/（μg/mL）	LOQ/（μg/mL）	Standard working curve	R²	RSD/%
BCA	2.65-2 648	1.0	y=0.001 423x+0.249 1	0.886	5.44
UV_{258 nm}	0.88-4 413	0.5	y=0.001 170x+0.121 3	0.998	1.10

Group		Min/d	Q₁/d	Q₂/d	Q₃/d	Max/d	Mean/d	E/%	L-test	W-test
CON		5	44	67	77	126	62	—	—	—
PIRA		5	50	80	94	135	74	19.7	****	****
ρ （FNPs） / （μg/mL）	0.25	5	45	80	84	120	67	8.3	***	****
	0.5	5	47	75	84	115	67	8.9	**	***
	1	5	50	80	85	106	68	10.4	****	****
	2.5	10	45	76	92	106	71	14.8	****	****
	5	5	57	80	93	131	74	19.2	****	****
	10	5	50	76	85	120	68	9.9	****	****

The anti-aging potential of peptides from fermented Nannochloropsis sp.

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