高效液相色谱法-蒸发光散射检测器测定表面活性剂中月桂酰基甘氨酸钠含量

doi:10.3969/j.issn.1001-1803.2023.02.013

Abstract

Abstract:

A method for rapid quantitative analysis of sodium lauroyl glycinate （SLG） was established by using a reversed-phase HPLC combined with evaporative light scattering detection （ELSD） without complex pre-treatment. HPLC-ELSD was equipped with Thermo Scientific Acclaim Surfactant Plus column （250 mm×4.6 mm, 5 μm）. Acetonitrile-0.1 mol/L ammonium acetate aqueous solution （the pH was adjusted to 5.4 by acetic acid） was used as the mobile phase for gradient elution and the parameters in ELSD were optimized as follows: Evaporation temperature was 75 ℃; nebulization temperature was 85 ℃; N₂flow rate was 0.9 L/min. The facial cleanser samples were extracted by mixed solvent of 80% acetonitrile-20% water and the amount of SLG was determined. The results showed that, in the range of 10-200 mg/L, the logarithm of the response value of ELSD was linearly related to the logarithm of the concentration of SLG with the correlation coefficients greater than 0.999, and the detection limit was 3 mg/L. The relative standard deviation of facial cleansing sample in 24 h was less than 1.5%, and the relative standard deviation in parallel samples was less than 2.0%. The average spike recoveries varied from 90.9% to 100.4% with standard addition. The developed HPLC-ELSD method could rapidly determine the content of SLG in facial cleansers with the advantages of simple operation, high accuracy and good reproducibility, and it was suitable for determination of other N-acylamino acid surfactants.

Key words: sodium lauroyl glycinate, HPLC, ELSD, N-acylamino acid

CLC Number:

TQ423

Zhuo Wenshan, Feng Shunqing, Tang Jianfeng. Determination of sodium lauroyl glycinate by HPLC-ELSD[J].China Surfactant Detergent & Cosmetics, 2023, 53(2): 220-225.

Figures/Tables 11

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References 14

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Time/min	0.1 mol/L Ammonium acetate aqueous solution/%	Acetonitrile/%
0.0	60	40
7.0	15	85
14.0	15	85
14.1	60	40
20.0	60	40

Extraction solvent		Area
Acetonitrile/%	Water/%	Area
80	20	3 932.8
60	40	3 922.0
40	60	3 132.1
20	80	3 179.1

Gas flow rate/ （L/min）	Area	Temperature of evaporation/℃	Area	Temperature of nebulization/℃	Area
0.9	3 527.6	75	3 499.8	85	3 527.6
1.2	2 622.3	65	3 130.1	75	3 275.7
1.5	1 488.3	55	2 547.9	65	3 164.7
1.8	892.2	45	1 181.9	55	3 107.0

Mass concentration of SLG/ （mg/L）	Area	Average area	Area RSD/%	Average RT/min	RT RSD/%
100	3 200.8	3 199.4	1.8	8.560	0.041
	3 159.4
	3 287.4
	3 211.1
	3 138.3
200	8 064.8	8 025.1	1.9	8.564	0.10
	7 769.4
	8 044.4
	8 109.6
	8 137.1

Background of SLG in facial cleanser/（mg/L）	Added/ （mg/L）	Found/ （mg/L）	Recovery/ %	Average recovery/ %	RSD/ %
43.0	10	53.8	101.4	97.4	4.0
	20	63.5	100.8
	50	92.1	99.0
	100	135.0	94.7
	150	185.3	90.9

Determination of sodium lauroyl glycinate by HPLC-ELSD

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Sample NO.	Weight of facial cleanser/mg	Mass concentration/（mg/L）	Weight percentage of SLG/%	RSD/ %
1	43.78	110	2.51	1.9
2	42.21	104	2.47
3	44.01	110	2.50
4	47.18	118	2.51
5	49.50	129	2.60

Sample NO.	Mass concentration of SLG/（mg/L）					RSD/%
Sample NO.	0 h	10 h	15 h	20 h	24 h	RSD/%
1	106	109	108	109	110	1.3
2	118	118	120	122	118	1.5
3	125	128	129	130	129	1.5

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