高效液相色谱法测定追风活血膏中8种成分

doi:10.3969/j.issn.1001-1803.2022.12.012

Abstract

Abstract:

A high performance liquid chromatographic （HPLC） method was developed for determination of the eight components in Zhuifeng Huoxue Ointment, namely, brucine, aconitine, strychnine, angelica, frankincense, myrrh, ecdysterone and sinomenine. The chromatographic column was an Agilent ZORBAXSB-C₁₈ column （4.6 mm×250 mm, 5 μm）; the mobile phase was methanol （phase A） and 0.1% aqueous solution of H₃PO₄ （phase B）; the flow rate was 1.0 mL/min; the column temperature was room temperature; the detection wavelength was 256 nm. The average recoveries ranged from 98.74%-99.67%, and the relative standard deviations （RSDs） were in the range of 0.85%-1.76%. The content of strychnine, aconitine, brucine, angelica, frankincense, myrrh, ecdysterone and sinomenine in the five batches of samples were 0.031-0.349, 0.021-0.166, 0.024-0.298, 0.021-0.219, 0.017-0.186, 0.015-0.158, 0.015-0.149, 0.016-0.179 mg/g, respectively. The HPLC method developed is simple, stable and controllable, and can be used for the quality control of Zhuifeng Huoxue Ointment.

Key words: Zhuifeng Huoxue Ointment, high performance liquid chromatography, quality control

CLC Number:

TQ658

Wan Liujing,Zhang Li. Determination of 8 components in Zhuifeng Huoxue Ointment by high performance liquid chromatography[J].China Surfactant Detergent & Cosmetics, 2022, 52(12): 1342-1347.

Figures/Tables 4

Fig. 1

Tab. 1

Tab. 2

Tab. 3

References 17

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成分	回归方程	线性范围/（μg/mL）	r
士的宁	Y=15.014+274.516X	0.059~0.592	0.999 9
乌头碱	Y=732.026-15.781X	0.131~1.310	0.999 9
马钱子碱	Y=167.127+478.137X	0.118~1.180	0.999 8
当归	Y=237.514+115.241X	0.071~0.714	0.999 9
乳香	Y=72.241+173.320X	0.079~0.792	0.999 8
没药	Y=632.843+392.173X	0.091~0.914	0.999 9
蜕皮甾酮	Y=184.243+112.834X	0.174~1.740	0.999 8
青藤碱	Y=461.367+228.258X	0.068~0.684	0.999 9

成分	称取量/g	样品中含量/mg	加入量/mg	测得量/mg	回收率/%	平均回收率/%	RSD/%
士的宁	2.048	0.332	0.334	0.666	99.18	99.31	0.17
	2.194	0.337	0.334	0.671	99.46
	2.004	0.338	0.334	0.672	99.52
	1.991	0.333	0.334	0.667	99.09
	1.994	0.335	0.334	0.669	99.35
	1.993	0.330	0.334	0.663	99.26
乌头碱	2.018	0.185	0.185	0.370	98.54	98.74	0.19
	2.015	0.181	0.185	0.366	98.68
	2.020	0.190	0.185	0.375	98.97
	1.995	0.190	0.185	0.374	98.69
	1.991	0.182	0.185	0.367	98.62
	1.990	0.182	0.185	0.367	99.04
马钱子碱	2.020	0.214	0.214	0.428	99.18	99.25	0.12
	2.012	0.214	0.214	0.428	99.34
	2.095	0.216	0.214	0.430	99.76
	2.038	0.215	0.214	0.429	99.09
	1.999	0.213	0.214	0.427	98.93
	1.998	0.212	0.214	0.426	99.79
当归	2.017	0.102	0.103	0.205	99.84	99.67	0.17
	2.028	0.102	0.103	0.205	99.15
	2.075	0.103	0.103	0.206	99.32
	2.086	0.106	0.103	0.209	99.84
	1.997	0.105	0.103	0.208	99.69
	1.991	0.100	0.103	0.203	100.18
乳香	2.083	0.082	0.084	0.166	99.14	99.36	0.15
	2.049	0.086	0.084	0.169	99.51
	2.094	0.082	0.084	0.166	99.98
	1.996	0.084	0.084	0.167	99.09
	1.999	0.085	0.084	0.168	99.12
	1.991	0.083	0.084	0.167	99.32
没药	2.020	0.090	0.092	0.181	98.74	98.79	0.14
	2.097	0.091	0.092	0.183	98.57
	2.047	0.093	0.092	0.185	99.13
	2.096	0.095	0.092	0.186	98.21
	1.993	0.092	0.092	0.184	98.85
	1.996	0.089	0.092	0.181	99.24
蜕皮甾酮	1.991	0.090	0.088	0.178	98.47	99.18	0.21
	1.998	0.087	0.088	0.175	99.13
	1.997	0.086	0.088	0.175	99.42
	1.998	0.087	0.088	0.175	99.97
	2.017	0.090	0.088	0.178	98.85
	2.085	0.091	0.088	0.179	99.24
青藤碱	2.066	0.147	0.151	0.298	99.17	99.54	0.16
	2.016	0.152	0.151	0.303	99.51
	1.999	0.152	0.151	0.303	99.56
	1.995	0.150	0.151	0.301	99.74
	1.995	0.150	0.151	0.301	99.38
	1.999	0.155	0.151	0.306	99.88

成分	含量/（mg/g）
	批号100801		批号100903		批号101101		批号101201		批号101205
	醇提	油炸	醇提	油炸	醇提	油炸	醇提	油炸	醇提	油炸
士的宁	0.343	0.032	0.348	0.031	0.345	0.032	0.349	0.031	0.346	0.031
乌头碱	0.166	0.021	0.165	0.021	0.161	0.021	0.159	0.021	0.164	0.021
马钱子碱	0.297	0.025	0.291	0.025	0.294	0.024	0.298	0.024	0.292	0.025
当归	0.215	0.022	0.211	0.021	0.210	0.021	0.219	0.022	0.216	0.022
乳香	0.186	0.018	0.184	0.017	0.182	0.017	0.187	0.017	0.185	0.018
没药	0.151	0.016	0.157	0.016	0.154	0.015	0.152	0.015	0.158	0.015
蜕皮甾酮	0.148	0.015	0.141	0.016	0.142	0.016	0.149	0.015	0.144	0.016
青藤碱	0.172	0.017	0.170	0.016	0.179	0.016	0.175	0.017	0.176	0.016

Determination of 8 components in Zhuifeng Huoxue Ointment by high performance liquid chromatography

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