丹参水溶性提取物丹参酚酸B的制备及应用

doi:10.3969/j.issn.1001-1803.2022.10.010

Abstract

Abstract:

The purpose was to study the preparation and biotransformation application of salvianolic acid B, a water-soluble extract of Salvia miltiorrhiza. The extraction rate and extraction rate of salvianolic acid B were used as evaluation indicators to compare the water-soluble extraction methods of Salvia miltiorrhiza, including water decoction reflux method, ultrasonic extraction method, percolation method, etc., which were determined by high performance liquid chromatography （HPLC）. After repeated exploration and comparison, the study finds that the extraction rate of salvianolic acid B in the conventional water decoction reflux method is as high as 72.59%, which is much higher than of the ultrasonic extraction method of 63.72% and the percolation method of 65.69% （P<0.05）. In the application of biotransformation of salvianolic acid B, the study finds that 100 g of salvianolic acid B can yield 14.2 g of Danshensu （purity≥95%） and 35.8 g of Prewalskinic acid A （purity≥91%）, which indicates that the biotransformation is very successful. In this study, we have conducted related research on the preparation process of salvianolic acid B, a water-soluble extract of Salvia miltiorrhiza, obtained a simple, efficient and scientific process route, and studied its biotransformation application, which found a high conversion efficiency of salvianolic acid B into Przewalskinic acid A and Danshensu. This research lays a theoretical and practical foundation for the further development and practical application of Salvia miltiorrhiza.

Key words: Salvia miltiorrhiza, decocting and refluxing method, salvianolic acid B, Danshensu

CLC Number:

TQ658

Chen Pingping,Xu Quansheng,Zhao Lina,Zhang Xiaoxia. Study on the preparation and application of salvianolic acid B, a water-soluble extract of Salvia miltiorrhiza[J].China Surfactant Detergent & Cosmetics, 2022, 52(10): 1100-1106.

Figures/Tables 13

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References 20

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回流时间/min	30	60	90	120
提取率/%	61.25	67.34	72.59	68.46
出膏率/%	58.31	63.06	61.02	59.28

超声时间/min	30	60	90	120
提取率/%	52.18	59.29	63.72	57.32
出膏率/%	44.37	51.25	55.46	51.08

渗漉速度/（d/s）	1	3	5	7
提取率/%	56.36	61.25	65.69	62.83
出膏率/%	46.72	52.48	58.47	53.54

	酸沉前	酸沉后
ρ（丹参酚酸B）/（mg/mL）	1.562	1.559
丹参酚酸B纯度/%	7.51	8.86
出膏率/%	48.94	40.65

树脂型号	比表面积/（m²/g）	粒径/mm	平均孔径/nm	极性
X-5	500~600	0.2~0.6	290~300	非极性
D101	≥400	0.3~1.3	100~110	非极性
YWD06	≥300	0.3~1.3	155~165	极性
YWD07	≥250	0.3~1.3	150~160	极性
HPD450	500~550	0.3~1.3	90~110	弱极性
HPD600	500~550	0.3~1.3	100~120	极性
LD605	150~200	0.3~1.3	140	非极性
D160	200~300	0.3~1.3	100	强极性

Study on the preparation and application of salvianolic acid B, a water-soluble extract of Salvia miltiorrhiza

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References 20

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树脂型号	吸附容量/（mg/mL）
X-5	25.07
D101	15.72
YWD06	30.74
YWD07	24.13
HPD450	18.14
HPD600	16.72
LD605	13.15
D160	15.28