Abstract:

Staphylococcus aureus was used as the test bacteria, and the 3 cm×3 cm JB-00 cotton cloth was used as the carrier. The staining carrier was prepared by inoculating 20 μL of the test bacteria suspension on each piece of cotton cloth by the 5-point method. 8 kinds of commercially available fabric detergents were used as the samples to be tested. Sterile hard water was used as the diluent to dilute the sample to be tested in a wide-mouth bottle to an effective concentration. 3 pieces of staining carrier were added to each wide-mouth bottle and washed for 20 minutes with magnetic stirring at 200 r/min, and then the water was changed and they were rinsed for 2 times and 5 min each time, which simulated the actual washing process of the fabric. Finally, the sterilization rate of different products was calculated by the plate counting method. The results show that the sterilization rate of samples A, G and H is above 95%, the sterilization rate of sample B is above 80%, the sterilization rate of samples C and E is above 60%, the sterilization rate of sample D is above 30%, and the sterilization rate of sample F is below 30%. This method can effectively distinguish the sterilization performance of different samples （P<0.05）; The stability of sterilization performance evaluated by this method is related to the level of the sterilization rate of the sample. For samples A, G and H that have good sterilization effects, the stability of sterilization rate is also high, while for samples D and F that have poor sterilization effects, the stability of sterilization rate is also low. Therefore, this method can be used as an evaluation method for the sterilization performance of fabric detergents.

Key words: fabric detergent, Staphylococcus aureus, sterilization performance, evaluation method