黄杜中药漱口液对牙周病相关菌的影响及作用机理研究

doi:10.3969/j.issn.1001-1803.2022.01.004

Abstract

Abstract:

The effect and mechanism of Huangdu Chinese herbal mouthwash on the activity of periodontal disease-related bacteria were explored. Periodontal disease-related pathogen Porphyromonas gingivalis and probiotic Weissella cibaria were selected as the indicator bacteria. The effect of Huangdu Chinese herbal mouthwash on the activity of P. gingivalis and W. cibaria was investigated by microbroth dilution method. From the perspective of the cell structure, the regulatory mechanism of Huangdu mouthwash on these two indicators was further investigated by detecting the integrity of cell wall and cell membrane and the activity of energy-metabolism enzymes. The results show that Huangdu mouthwash has significant inhibitory effect on pathogenic bacteria P. gingivalis （growth rate in stable period <20%）, and almost no inhibition on probiotic W. cibaria （growth rate in stable period >80%）. While cetylpyridinium chloride mouthwash significantly inhibits the growth of pathogen P. gingivalis and probiotic W. cibaria, which is almost in the lag phase （growth rate <10%） within 24 hours. Compared with the control group, the content of extracellular AKP, K⁺, nucleic acid and protein of pathogenic bacteria P. gingivalis is significantly increased （P<0.01）, while the activities of energy-metabolism enzymes SDH and MDH are significantly decreased by 19.84% and 10.31% （P<0.01）, but no significant effect on W. cibaria （P>0.05） is observed in the case of Huangdu mouthwash. It can be concluded that Huangdu mouthwash has bacteriostatic effect by destroying the integrity of cell membrane and cell wall of P. gingivalis and disturbing the energy-metabolism system, but it has little effect on probiotic W. cibaria. However, cetylpyridinium chloride mouthwash has both reduce the activities of pathogen and probiotic by destroying cell membrane and cell wall. The present work suggests that the Huangdu Chinese herbal mouthwash can increase the relative proportion of oral probiotics, improve oral flora, and thus provide a feasible reference for the prevention and treatment of periodontal disease.

Key words: Chinese herbal mouthwash, periodontal disease, antibacterial mechanism, Porphyromonas gingivalis, Weissella cibaria, probiotic

CLC Number:

TQ658

Wang Xiaoyu,Wang Yajing,Zhao Xin,Wang Yanwen,Wang Yu,Huang Zanyang. Study on the effect and mechanism of Huangdu Chinese herbal mouthwash on periodontal disease-related bacteria[J].China Surfactant Detergent & Cosmetics, 2022, 52(1): 20-27.

Figures/Tables 6

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Study on the effect and mechanism of Huangdu Chinese herbal mouthwash on periodontal disease-related bacteria

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