高效液相色谱法测定化妆品中的溴米索伐、卡溴脲和卡立普多及质谱确证

doi:10.3969/j.issn.1001-1803.2021.06.014

Abstract

Abstract:

An analytical method for the determination of bromisoval, carbromal and carisoprodol in cosmetics was developed based on high-performance liquid chromatography （HPLC）. This method was capable of simultaneously quantifying the three analytes in a single run by combining a diode array detector （DAD） and an evaporative light scattering detector （ELSD） in series. The limits of quantitation （LOQs） for bromisoval and carbromal were 5 mg/kg, and the average recoveries ranged from 90.7% to 100.5% with the relative standard deviations （RSDs） less than 5.8%. The LOQ for carisoprodol was 20 mg/kg, and the average recoveries ranged from 96.8% to 103.9% with the RSDs less than 5.9%. The suspicious samples were subjected to further verification with high-performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS）. This method is simple, fast and suitable for the routine analysis of bromisoval, carbromal and carisoprodol in cosmetics.

Key words: cosmetics, bromisoval, carbromal, carisoprodol, high-performance liquid chromatography, mass spectrometric verification

CLC Number:

TQ658

SHANG Yu-han,GUO Xiang-yu,ZONG Yi-jing,LI Tao,LI Wen-tao,MA Qiang. Determination of bromisoval, carbromal and carisoprodol in cosmetics using HPLC and their verification by HPLC-MS/MS[J].China Surfactant Detergent & Cosmetics, 2021, 51(6): 564-568.

Figures/Tables 5

Fig. 1

Tab. 1

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Tab. 2

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References 20

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待测物	保留时间/min	定性离子对（m/z）	锥孔电压/V	碰撞能量/eV	相对离子丰度/%	允许相对偏差/%
溴米索伐	1.83	223.6/179.9,223.6/57.1	20	14, 18	100,47	±25
卡溴脲	2.27	237.2/194.1,237.2/114.2	30	8, 14	100,80	±20
卡立普多	2.47	261.1/176.1,261.1/62.0	30	6, 15	100,81	±20

化合物	w/（mg·kg^-1）	水剂类化妆品		乳液类化妆品		膏霜类化妆品
化合物	w/（mg·kg^-1）	回收率/%	RSD/%	回收率/%	RSD/%	回收率/%	RSD/%
溴米索伐	5	99.4	1.6	90.7	2.5	97.4	2.0
	10	99.8	1.0	91.6	2.9	99.7	3.4
	50	100.1	1.3	92.7	3.4	98.9	4.6
卡溴脲	5	94.6	3.3	98.7	3.4	96.4	5.8
	10	94.6	3.2	98.9	3.3	100.5	3.9
	50	94.0	3.8	98.9	2.1	95.2	2.6
卡立普多	20	96.8	5.0	102.7	4.5	103.6	3.1
	40	99.7	4.8	103.6	4.2	102.5	3.0
	200	99.1	5.8	103.3	5.9	103.9	4.6

Determination of bromisoval, carbromal and carisoprodol in cosmetics using HPLC and their verification by HPLC-MS/MS

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