祛痘中草药的筛选及其在治疗痤疮中的应用

doi:10.3969/j.issn.1001-1803.2021.06.008

Abstract

Abstract:

The anti-acne efficacy of herbs was evaluated according to their antibacterial effect and their inhibitory effect on 5α-reductase. Scutellaria Baicalensis, Salvia miltiorrhiza and Forsythia were thus screened from the Chinese herbs. An anti-acne gel was prepared in which the alcohol extracts of the three herbs were used as effective ingredients and its clinical effect was investigated. The minimum inhibitory concentrations （MIC） of the compound formula on P. acnes and S. aureus are 3.13 and 3.13 mg/mL, respectively. The inhibition rate against 5α-reductase at 5 mg/mL is 46.5%. This anti-acne gel can effectively reduce the severity of acne. The results indicate that the extracts of these three herbs show good antibacterial effect and enzyme inhibitory effect, and they have good application effect.

Key words: antibacterial, 5α-reductase, clinical, Chinese herb

CLC Number:

TQ658

ZHAO Da-min,WANG Xue-dong,ZHAI Chun-tao. Screening of anti-acne herbs and their application in the treatment of acne[J].China Surfactant Detergent & Cosmetics, 2021, 51(6): 522-527.

Figures/Tables 12

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References 21

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	空白管/mL	药品管/mL	终浓度
5α-还原酶提取液	0.2	0.2
睾酮（T）	0.01	0.01	100 μmol/L
NADPH	0.1	0.1	150 μmol/L
药物样品	—	0.1	5 mg/mL
70%乙醇	0.1	—
磷酸盐缓冲液	0.6	0.6

组别	成分	质量分数/%
A组	去离子水	至100
	1, 3-丁二醇	2.00
	EDTA-2Na	0.05
	U21	0.40
B组	精氨酸（10%）	3.50
C组	对羟基苯乙酮	0.50
	1, 2-己二醇	0.50
	水	5.00
D组	中药复方	5.00

样品	科	痤疮丙酸杆菌ATCC 6919	金黄色葡萄球菌ATCC 6538	5α-还原酶抑制率/ %
土茯苓	百合科	1	3	-8.4
连翘	木犀科	3	4	20.5
黄芩	唇形科	4	3	19.8
啤酒花	桑科	1	2	13.2
紫草	紫草科	3	1	7.8
蒲公英	菊科	1	3	9.4
葡萄叶	葡萄科	3	2	9.4
苦参	豆科	3	3	16.1
茯苓	多孔菌科	2	1	5.4
穿心莲	爵床科	2	2	3.2
覆盆子	蔷薇科	1	3	6.3
栀子	茜草科	1	1	3.4
天冬	百合科	3	0	11.9
麦冬	百合科	2	1	13.5
白柳皮	杨柳科	3	2	15.2
丹参	唇形科	4	3	28.6

样品	MIC/（mg·mL^-1）		5α-还原酶抑制率/%
样品	P. acnes	S. aureus	5α-还原酶抑制率/%
丹参提取物	12.50	7.81	28.6
黄芩提取物	50	25	19.8
连翘提取物	50	6.25	20.5
中药复方	3.13	3.13	46.5

使用天数	缓解人数占比/%
使用天数	3 d	7 d	14 d	21 d	28 d
严重程度	73.91	69.57	95.65	91.30	95.65
面积	69.57	73.91	86.96	73.91	86.96
发红情况	60.87	78.26	82.61	86.96	82.61
肿胀情况	65.22	73.91	78.26	78.26	78.26
疼痛情况	47.83	52.17	86.96	73.91	73.91

Screening of anti-acne herbs and their application in the treatment of acne

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