常用表面活性剂对唾液链球菌与食窦魏斯氏口腔益生菌的影响

doi:10.3969/j.issn.1001-1803.2021.05.004

Abstract

Abstract:

The effects of different mass concentrations of surfactants on the growth of oral probiotics were studied. Six surfactants commonly used in oral care products, i.e. PEG-40 hydrogenated castor oil （RH-40） , PEG-60 hydrogenated castor oil （RH-60） , cocamidopropyl betaine （CAB-35） , sodium lauroyl sarcosinate （LS-97） ,sodium cocoyl glycinate （AK-301） and sodium dodecyl sulfate （SDS） , were used; Weissella cibaria and Streptococcus salivarius were used as experimental probiotic strains. The results of modified Oxford cup method showed that probiotics were all sensitive to the six surfactants with a mass concentration of 10 g/L; the results of broth micro-dilution method showed that different mass concentrations of different surfactants had different effects on oral probiotics. RH-60 with a mass concentration ≤0.078 g/L and RH-40 with a mass concentration ≤0.156 g/L could promote the growth of probiotics; RH-60 with a mass concentration of 0.156-0.625 g/L, CAB-35 with a mass concentration of 0.02 g/L, and LS-97 and AK-301 with mass concentrations ≤0.039 g/L had no significant effect on the growth of probiotics; SDS, LS-97 and AK-301 with mass concentrations ≥0.313 g/L and CAB-35 with a mass concentration ≥1.25 g/L completely inhibited the growth of probiotics. The inhibitory effects of RH-60 and RH-40 with mass concentrations >0.156 g/L on probiotics were weaker than other surfactants at the same mass concentration. Low mass concentrations of AK-301, CAB-35 and LS-97, and each mass concentration of RH-60 and RH-40, could be given priority to be used in oral care products.

Key words: surfactant, Weissella cibaria, Streptococcus salivarius, probiotic, time-growth curve

CLC Number:

TQ423

WANG Yan-wen,WANG Ya-jing,ZHAO Xin,GAO Di,YE Xiang-yin,HUANG Zan-yang,WANG Xiao-yu,WANG Yu. Effects of commonly used surfactants on Streptococcus salivarius and Weissella oral probiotics[J].China Surfactant Detergent & Cosmetics, 2021, 51(5): 396-401.

Figures/Tables 5

Tab. 1

Fig. 1

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抑菌物质	抑菌圈直径/mm		敏感性
抑菌物质	W.cibaria	S.salivarius	敏感性
阳性对照	24.09±0.67^**	26.93±0.81^**	极敏
阴性对照	0	0	/
RH-60	7.92±0.07	7.85±0.05	低敏
RH-40	7.99±0.19	7.88±0.08	低敏
SDS	16.03±0.20^**	17.04±0.31^**	高敏
LS-97	16.33±0.15^**	15.25±0.40^**	高敏
CAB-35	15.24±0.15^**	16.15±0.24^**	高敏
AK-301	15.68±0.12^**	15.95±0.21^**	高敏

Effects of commonly used surfactants on Streptococcus salivarius and Weissella oral probiotics

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