用于化妆品功效评价的皮肤细胞类型、特点及应用概况

doi:10.3969/j.issn.1001-1803.2021.01.009

Abstract

Abstract:

The characteristics of keratinocytes （KC and HaCaT）, melanocytes （MC）, mouse melanoma cells （B-16） , mononuclear macrophages （THP-1） and human skin fibroblasts （HSF） were reviewed, as well as their applications in cosmetic efficacy evaluation. The commonly used detection indexes for evaluating the efficacy of different types of cosmetics by use of these cells were summarized. The characteristics of these skin cells and their advantages and disadvantages in vitro culture were analyzed, which might provide a theoretical basis for evaluating the efficacy of cosmetic raw materials.

Key words: skin cell, cosmetic, efficacy evaluation

CLC Number:

TQ658

MA Yu-chen,LIU Lei,HE Cong-fen. Types, characteristics and application of the skin cells used in cosmetic efficacy evaluation[J].China Surfactant Detergent & Cosmetics, 2021, 51(1): 50-55.

Figures/Tables 4

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References 42

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细胞类型	位置	特点	优点	缺点	替代细胞	功效评价类型及检测指标
KC	表皮层	可产生大量细胞因子、粘多糖和细胞角蛋白等物质,以维持皮肤的渗透作用及屏障功能	生长较快、细胞存活率较高	易老化,传代次数较少	HaCaT	保湿功效评价：AQP3、TJP、FLG、HAS
KC	表皮层	可产生大量细胞因子、粘多糖和细胞角蛋白等物质,以维持皮肤的渗透作用及屏障功能	生长较快、细胞存活率较高	易老化,传代次数较少	HaCaT	抗氧化功效评价：ROS、SOD、CAT、GSHPX、MDA、caspase

细胞类型	位置	特点	优点	缺点	功效评价类型及检测指标
HSF	真皮层	具有蛋白质合成能力,真皮层最主要细胞	分布广泛、取材方便,对机体损伤较小、体外培养容易成活、增殖较快	易老化,传代次数较少	抗衰老功效评价：细胞增殖、ROS、SOD、CAT、GSHPX、Ⅰ型胶原蛋白、MMP、β-半乳糖苷酶
HSF	真皮层	具有蛋白质合成能力,真皮层最主要细胞	分布广泛、取材方便,对机体损伤较小、体外培养容易成活、增殖较快	易老化,传代次数较少	创面愈合：HSF迁移率、DNA

Types, characteristics and application of the skin cells used in cosmetic efficacy evaluation

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