表面活性剂辅助超声萃取杜仲叶总多酚及其抗氧化活性研究

doi:10.3969/j.issn.1001-1803.2020.03.005

Abstract

Abstract:

The surfactant-assisted ultrasonic extraction of total polyphenols from Eucommia ulmoides leaves was optimized. The antioxidant activity in vitro was compared between the extracts from Eucommia ulmoides leaves with different extraction processes. Firstly, on the basis of the single-factor test, the total polyphenols were used as indicators to optimize the extraction conditions by the orthogonal experiment. Then the antioxidant activity of the extract from Eucommia ulmoides leaves was evaluated in vitro in terms of the hydroxyl radical scavenging rate, ABTS free radical scavenging rate, DPPH free radical scavenging rate and reducing power. The experimental results showed that the optimal extraction process was SDS dosage of 0.1 g/L, ultrasonic extraction time of 5 min, ethanol volume fraction of 50%, solid-liquid ratio of 1∶20 （g∶mL）, and temperature of 45 °C. Under this condition, the total polyphenol yield of Eucommia ulmoides leaves was 6.770%. The extract from Eucommia ulmoides leaves had certain antioxidant activity in vitro.

Key words: Eucommia ulmoides leaf, total polyphenols, extraction process, surfactant, antioxidation

CLC Number:

TQ658

Lu-jun LI,Zhi-hong WANG,Yan-ping HUO,Xiang WANG,Qiu-ling YANG,Mi-jun PENG. Study on surfactant-assisted ultrasonic extraction of total polyphenols from Eucommia ulmoides leaves and their antioxidant activity[J].China Surfactant Detergent & Cosmetics, 2020, 50(3): 164-170.

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References 12

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水平	A 乙醇体积分数/%	B 料液比/（g·mL^-1）	C 温度/℃
1	30	1∶15	35
2	50	1∶20	40
3	70	1∶25	45

表面活性剂	类型	杜仲总多酚得率/%
吐温20	非离子型表面活性剂	6.224
聚乙二醇	非离子型表面活性剂	6.219
十二烷基硫酸钠	阴离子型表面活性剂	6.463
十二烷基磺酸钠	阴离子型表面活性剂	6.445
十六烷基三甲基溴化铵	阳离子型表面活性剂	6.342
空白		6.217

因素	A	B	C		得率/%
实验1	1	1	1	1	5.220
实验2	1	2	2	2	5.430
实验3	1	3	3	3	5.560
实验4	2	1	2	3	6.100
实验5	2	2	3	1	6.770
实验6	2	3	1	2	6.450
实验7	3	1	3	2	6.600
实验8	3	2	1	3	6.360
实验9	3	3	2	1	6.580
均值1	5.403	5.973	6.010	6.190
均值2	6.440	6.187	6.037	6.160
均值3	6.513	6.197	6.310	6.007
极差水平	1.110 A₃	0.224 B₃	0.300 C₃	0.183 D₁

因素	偏差平方和	自由度	F比	F临界值	显著性
乙醇体积分数	2.312	2	39.86	19.00	*
料液比	0.095	2	1.638	19.00
温度	0.165	2	2.845	19.00

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Study on surfactant-assisted ultrasonic extraction of total polyphenols from Eucommia ulmoides leaves and their antioxidant activity

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