藏荆芥提取物对酪氨酸酶活性及其动力学研究

doi:10.3969/j.issn.1001-1803.2019.05.007

Abstract

Abstract:

The effect of extract of Nepeta angustifolia C.Y.Wu on tyrosine monophenolase and diphenolase activity and inhibition kinetics were studied by enzymatic reaction using L-tyrosinase and L-DOPA as substrates. Experimental results show that the extract of Nepeta angustifolia C.Y.Wu, has inhibitory effect on tyrosine monophenolase and diphenolase, and the concentrations of the extract of Nepeta angustifolia C.Y.Wu leading to 50% decrease in monophenolase and diphenolase activity（IC₅₀）are calculated to be 0.138 8 and 0.533 2 g/L, respectively. The inhibition of tyrosine diphenolase by the extract of Nepeta angustifolia C.Y.Wu is a reversible process. The Lineweaver-Burk plots show that the type of action is competitive inhibition with an inhibition constant of 504.846 mg/L. The extract of Nepeta angustifolia C.Y.Wu has a significant inhibitory effect on tyrosinase and has the potential to be developed into a natural tyrosinase inhibitor.

Key words: cosmetic additive, extract of Nepeta angustifolia C.Y.Wu, tyrosinase, kinetics, reversible competitive inhibition

CLC Number:

TQ658

WANG Fei-fei,WANG Min-zhu,WANG Han,HUANG Shan,DANZENG Jiang-bai,LI Bin. The effect of extract of Nepeta angustifolia C.Y.Wu on tyrosinase activity and kinetics[J].China Surfactant Detergent & Cosmetics, 2019, 49(5): 315-319.

Figures/Tables 7

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References 16

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反应液的组成	V/μL
反应液的组成	a	b	c	d
底物	10	10	10	10
溶剂	90	80	70	60
药品	–	–	20	20
酪氨酸酶	–	10	–	10

ρ（藏荆芥提取物）/ （mg·L^-1）	回归方程	R²	K_m/（mg·L^-1）
0	y=17.217x+13.365	0.993 1	1.288
100	y=18.039x+15.567	0.998 4	1.159
200	y=26.436x+11.840	0.998 7	2.233
300	y=26.356x+15.165	0.991 1	1.738
400	y=22.401x+20.754	0.995 0	1.079
500	y=32.365x+15.592	0.983 2	2.076

The effect of extract of Nepeta angustifolia C.Y.Wu on tyrosinase activity and kinetics

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