Abstract: An investigation of inhibitory effects of surfactant dodecyl hydroxypropyl sulfobetaine (DSB) against adhesion of Staphylococcus epidermidis biofilm formation bacteria was conducted in order to find possible new research direction and treatment method of S.epidermidis biofilm bacteria associated infection.Vancomycin is applied as positive control,XTT reduction assay is used to evaluate the effect of DSB on the initial adhesion of S.epidermidis.The presented results show that DSB displays a significantly inhibitory effect on the initial adhesion of S.epidermidis at the mass concentration of 64 mg/L.After the experimental starting materials are pretreated by DSB at the mass concentration of 512,256 and 128 mg/L,the initial adhesion of S.epidermidis is significantly inhibited.Therefore,experimental results indicate that DSB has an significantly inhibitory effects on the initial adhesion in the process of biofilm formation of S.epidermidis.

Key words: surfactant, dodecyl hydroxypropylsulfobetaine, adhesion, Staphylococcus epidermidis, biofilm