Abstract: An ultra-high performance liquid chromatography (UPLC) method was established for determination of formaldehyde in fragrance for household and personal products.After derived with 2,4-dinitrophenyl hydrazine (DNPH),the formaldehyde in the fragrance was determined by UPLC.The separation was performed on an Agilent ZORBAX Eclipse Plus C₁₈ column (2.1 mm×100 mm,1.8 μm).The mobile phase was consisted of acetonitrile and water (volume ratio of 60∶40) with a flow rate of 0.3 mL/min.The wavelength of UV detector was set at 352 nm.A good linearity of formaldehyde in the range of 0.1~10 mg/L is obtained and the detection limit is about 0.03 mg/L.The spiked recoveries are ranged within 96.3%~104.1%,with relative standard deviations of 0.2%~1.4% (n=3).10 batches of fragrance for household and personal products were determined using the established method and five batches are found containing formaldehyde.However,the content of formaldehyde in the samples is much lower than the limit specified by Hygienic Standard for Cosmetics.

Key words: fragrance for household and personal products, formaldehyde, ultra-high performance liquid chromatography, derivatization