Abstract: A high performance liquid chromatography-mass spectrometry (HPLC-MS/MS) method was established for simultaneous determination of 33 kinds of banned and/or restricted component in hair dyes.After extracted by mixed solution with methanol and 2 g/L ascorbic acid aqueous solution,purified by n-hexane,the samples were analyzed by HPLC-MS/MS.The separation was performed on an Agilent ZORBAX Extend C₁₈ column (2.1 mm×100 mm,1.8 μm) and gradient eluted with aqueous solution of 5 mmol/L ammonium acetate and acetonitrile.The electro spray ionization source in positive and negative ion mode was used for qualitative and quantitative analysis of the 33 components under the multiple reaction monitoring mode (MRM).The results show good linear relationships with all correlation coefficients higher than 0.99.The limits of detection for the 33 components are in the range of0.01-29.5 mg/kg.The average spiked recoveries of the 33 components are in the range of 71%-115%.

Key words: hair dyes, dye, high performance liquid chromatography-mass spectrometry