Abstract: Apoptosis state of fibroblast of adult skin can be reflected by mitochondrial membrane potential to a certain extent.Experimental results showed that,as comparing with result obtained in the normal control group,that in the hydrogen peroxide group shows reduction of the skin fibroblast mitochondrial membrane potential (P<0.01);while in the model groups with additive composed of extract of blended herb with separate dosages of 75,150 and 300 mg/L,their mitochondrial membrane potential of skin fibroblast are significantly increased (P<0.01),and shows a dose-dependent manner.Such experimental result indicated that the blended herb extract is effective for prevention of mitochondrial membrane potential decreasing caused by hydrogen peroxide and can be used for delaying of skin senility as a prophylactic measure.Hopefully,the blended herb extract may be used as an additive in cosmetics.

Key words: cosmetic additives, blended herb extract, mitochondrial membrane potential