Abstract: To develop the application of fermentation liquor of Ganoderma lucidum in the field of cosmetics,Saccharomyces ellipsoideus were used to acquire fermentation liquor Ganoderma lucidum,and the content of total polysaccharides,total polyphenols,total flavones and total polypeptides in the fermentation liquor were determined.The scavenging activity of OH·free radicals and DPPH free radicals as well as the influence on fibroblasts viability were measured to evaluate the possible anti-senility effect of the fermentation liquor.Finally,via in vitro tyrosinase activity test and intracellular tyrosinase activity test on melanin synthesis of mouses as well as melanin inhibition test,the evaluation for whitening effect of the fermentation liquor was conducted.Results showed that the fermentation liquor of Ganoderma lucidum contains 9.910 g/L of total polysaccharides,0.054 g/L of total polyphenols,0.045 g/L of total flavones,and 2.931 g/L of total polypeptides.The fermentation liquor with 25.89% and 42.18% volume fraction of fermentation liquor of Ganoderma lucidum could eliminate 50% OH·free radical and DPPH free radical.The fermentation liquor with 0.05%-0.2% volume fraction can promote fibroblasts proliferation effectively.The activity of tyrosinase is inhibited significantly by the fermentation liquor of Ganoderma lucidum and the inhibition rate reaches 73.22%.The fermentation liquor of Ganoderma lucidum could inhibit melanin synthesis by decreasing activity of tyrosinase.The fermentation liquor of Ganoderma lucidum exhibits efficacy for anti-senility and whitening.

Key words: cosmetic additive, Ganoderma lucidum, fermention, antioxidant, whitening