Abstract: Via the exploration of effect of the yeast extract that originated from rice wine production (WAE) on viability of fibroblast HS68 cells as well as its action for formation of melanin by melanoma B16 cells,the safety and efficacy of the WAE were examined.The results showed that,the safety WAE mass fraction for HS68 and B16 is ≤0.10%.As comparing with the control group,when mass fraction of WAE is 0.10% and 0.01%,it promotes HS68 cells proliferation to 106.36% and 109.33% respectively.The experiments demonstrated that under the safe concentration,WAE displays the effect of promoting proliferation of HS68 cells.WAE can inhibit 68.32%,51.68% and 19.77% of melanin formation when the WAE mass fraction is 0.10%,0.05% and 0.01% respectively.The experiments demonstrated that under the safety mass fraction,WAE displays the effect of inhibiting melanin formation of B16 cells.As comparing with Japanese beer yeast extract (A) and domestic beer yeast extract (B) with the same mass fraction,the safety and efficacy of WAE are superior to that of A and B.

Key words: additive of cosmetics, yeast extract, fibroblast, melanin, safety, efficacy