牛肝菌提取液在细胞水平的抗衰老功效研究

doi:10.13218/j.cnki.csdc.2015.09.007

Abstract

Abstract: Bolete extract with mass concentration of 60 g/L was obtained by water extraction method.Effects of bolete in the extract on mitochondrial activity of epidermal keratinous cells were detected with XTT colorimetry,as well as cell proliferation observed and effect on cellular DNA content of cell detected.Effects of bolete in the extract on cellular ROS content of human dermal fibroblast and ARE activation were detected with DCFH-DA assay,et al.Effects of bolete in the extract on expression of procollagen of type I in human dermal fibroblast was detected with immuno-fluorescence method.Results showed that the extract of bolete with mass concentration of 1.5 g/L can significantly increase the mitochondrial activity of epidermal keratinous cells and cellular DNA content as comparing with that of the control group and can apparently promote proliferation of cell.Further,ROS content in human dermal fibroblast is significantly reduced and ARE activation is promoted.Meanwhile,the extract of bolete can promote the expression of procollagen of type I too.

Key words: cosmetic additive, extract of bolete, anti-aging, antioxidation

CLC Number:

TQ658

FENG Bing, MA Lai-ji. Study of anti-aging efficacy of bolete at cell theory level[J].China Surfactant Detergent & Cosmetics, 2015, 45(9): 509-512.

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Study of anti-aging efficacy of bolete at cell theory level

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