Abstract: Dendrobium nobile prescriptions were extracted with distilled water and the mass concentration of Dendrobium nobile in the extract is 0.5 g·mL^-1.Effects of Dendrobium nobile in the extract on mitochondrial activity of epidermal keratinous cell were detected with XTT colorimetry,as well as cell proliferation observed and effect on cellular ATP contents of HaCaT cell detected.Effects of Dendrobium nobile in the extract on the inhibition of activity of melanin cell tyrosinase and formation of melanin were detected with dopa staining,et al.Effects of Dendrobium nobile prescriptions in the extract on expression of moisturizing related proteins in keratinous cell HaCaT was detected with immunofluorescence method.Results showed that the extracts of Dendrobium nobile prescriptions with mass concentration of 2.5 g·L^-1 can significantly increase the mitochondrial activity of epidermal keratinous cell and cellular ATP content as comparing with that of the control group and can apparently promote proliferation of HaCaT cell.Further,melanin content in melanophore is significantly reduced and activity of tyrosinase is inhibited without affecting cell state in vitro model of the melanocyte and HaCaT cell co-culture system.Meanwhile,the extract of Dendrobium nobile prescriptions also can promote the expression of the aquaglyceroporin of keratinous cell protein AQP3,tight junctions protein ZO-1 and Claudin-1.

Key words: Dendrobium nobile prescription, anti-aging, whitening, moisturizing