液相色谱-四极杆/离子阱串联质谱法测定牙膏中的绿原酸

doi:10.13218/j.cnki.csdc.2014.07.091

Abstract

Abstract: A comprehensive method for detection of chlorogenic acid in toothpaste was established with liquid chromatography coupled to quadrupole/linear ion trap tandem mass spectrometry (LC-QTRAP-MS/MS).The samples were extracted under ultrasonic assistance.The separation was carried out on a Kinetex C18 column (2.1 mm×100 mm,2.6 μm) using mobile phases of methanol-0.1% formic acid solution as eluant.A multiple reaction monitoring (MRM) in negative mode as survey scan and an enhanced production (EPI) scan as dependent scan in an information-dependent acquisition (IDA) experiment was adopted in mass spectrometry acquisition.External standard was employed for the quantification.Test results showed that content of the chlorogenic acid displays good linearity within 1.0～100 μg·L^-1 vs.the peak area of the chromatogram with a correlation coefficient (r) of 0.999 6.The limit of detection for the chlorogenic acid is 10.0 μg·kg^-1.The mean recoveries at the three spiked levels are 85.6%~96.6%,and the relative standard deviations are not higher than 5.8%.

Key words: toothpaste, liquid chromatography, quadrupole/linear ion trap tandem mass spectrometry, chlorogenic acid

CLC Number:

TQ658.4

MA Xiao-fei, SONG Wei, LIANG Tian-zuo, JIA Zhen-guo, FENG Hao-bin, ZHANG Yan. Detection of chlorogenic acid in toothpaste by liquid chromatography coupled to quadrupole/linear ion trap tandem mass spectrometry[J].China Surfactant Detergent & Cosmetics, 2014, 44(7): 406-409.

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Detection of chlorogenic acid in toothpaste by liquid chromatography coupled to quadrupole/linear ion trap tandem mass spectrometry

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