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胶束十二烷基硫酸钠增敏荧光法测定银杏叶片槲皮素含量
陈金娥, 张海容
忻州师范学院 生化分析技术研究所,山西 忻州 034000
摘要:
建立了银杏叶片中测定槲皮素含量的十二烷基硫酸钠(SDS)增敏荧光新方法。实验考察了表面活性剂种类、用量及pH等外界条件对槲皮素荧光强度的影响。结果表明,所选用的SDS、十六烷基三甲基溴化铵(CTMAB)、曲拉通X-100(Triton X-100)、β-环糊精(β-CD) 4种有序介质,SDS在碱性(pH=9.12)介质中对槲皮素荧光增敏效果最好,在最大激发波长464 nm、最大发射波长542 nm处有强荧光发射峰。在pH=9.12、SDS浓度为1.5×10-2 mol/L优化实验条件下,体系荧光强度与槲皮素含量成线性关系,线性范围为2.0~14 μg/mL,检出限为0.16 g/L,RSD为1.56%。本方法用于4种银杏叶片中槲皮素含量测定,结果满意。
关键词:  十二烷基硫酸钠  荧光光度法  槲皮素  银杏叶片
DOI:10.13218/j.cnki.csdc.2018.06.009
分类号:TQ423
基金项目:山西省科技厅软科学研究计划(2017041015-2);山西省教育厅1331化学优势学科(2017)
Determination of quercetin in Ginkgo leaves tablets by micellar SDS enhanced fluorimetry
Abstract:
A new method for the determination of quercetin in Ginkgo leaves tablets was established by sodium dodecyl sulfate (SDS) enhanced fluorimetry. The effects of external conditions, such as the type and amount of surfactant and pH on the fluorescence intensity of quercetin were investigated. Results show that among the four kinds of ordered media, i.e., SDS, CTMAB, Triton X-100 and β-CD, the fluorescence intensity of quercetin is greatly enhanced in SDS solution under alkaline condition (pH=9.12) with a strong fluorescence emission peak at λex/λem=464/542 nm. Under the optimal experimental conditions of pH=9.12 and SDS concentration of 1.5×10-2 mol/L the linear relationship between fluorescence intensity and concentration of quercetin is kept in the range of 2.0-14 μg/mL with the detection limit of 0.16 μg/L and RSD of 1.56%. The method has been applied to the determination of quercetin in four kinds of Ginkgo leaves tablets with satisfactory results.
Key words:  sodium dodecyl sulfate  fluorimetry  quercetin  Ginkgo leaves tablets

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