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日用化学工业:2018,Vol.>>Issue(6):330-335
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油茶籽油不皂化物的提取与抗氧化性研究
周振宇,杨成,蔡春辉
(江南大学 化学与材料工程学院,江苏 无锡 214122)
Study on extraction of unsaponifiable matter in camellia oil and its antioxidant activity
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中文摘要: 以油茶籽油为原料,通过皂化-己烷萃取法提取其不皂化物。通过气相色谱-质谱联用技术(GC-MS)对油茶籽油不皂化物的组成进行了分析,并通过1,1-二苯基-2-三硝基苯肼(DPPH)法、三价铁离子还原力(FRAP)法以及细胞抗氧化(CAA)法对其抗氧化性能进行了测定,并与α-生育酚进行了比较。结果表明,通过皂化-己烷萃取法得到的油茶籽油不皂化物主要含有烃类、三萜醇以及植物甾醇;油茶籽油不皂化物的DPPH自由基清除率可达到95%,EC50为1.9 g/L;油茶籽油不皂化物具有一定的Fe3+还原能力,在质量浓度为5 g/L时,FRAP值可达170;油茶籽油不皂化物能够进入细胞并对2,2’-偶氮(2-甲基丙基脒)二盐酸盐(ABAP)诱导的细胞内活性氧水平升高具有抑制作用,质量浓度为100 mg/L时CAA值可达36.6。
Abstract:Camellia oil was used as raw material to extract unsaponifiable matter (USM) by saponification-hexane extraction method. The compositions of USM in camellia oil were analyzed by gas chromatography-mass spectrometeand (GC-MS), and DPPH radical-scavenging assay, ferric reducing-antioxidant power (FRAP) test and cellular antioxidant activity assay (CAA) were applied to determine their antioxidant activity in comparison with α-tocopherol. Results indicate that the compositions of USM in camellia oil obtained by saponification-hexane extraction are mainly hydrocarbon, triterpene alcohol and phytosterol. The DPPH free radical scavenging rate of USM in camellia oil is 95%, and the EC50 is 1.9 g/L. USM in camellia oil has certain ability to reduce Fe3+, and the FRAP value is 170 at the concentration of 5 g/L. USM in camellia can enter the HSF cells and inhibit the ABAP-induced intracellular reactive oxygen species formation. The CAA value is up to 36.6, when the concentration of USM is 100 mg/L.
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