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日用化学工业:2017,Vol.>>Issue(7):408-413
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抗氧化菌的筛选及混菌发酵红景天条件优化
戴玥,余晓斌
(江南大学 生物工程学院 工业生物技术教育部重点实验室,江苏 无锡 214122)
Screening of antioxidant bacteria and optimization of conditions for fermentation of Rhodiola rosea with mixed bacteria
DAI Yue,YU Xiao-bin
(Key Laboratory of Industrial Biotechnology of the Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, Jiangsu, 214122, China)
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投稿时间:2017-03-29    
中文摘要: 通过测定12株酵母菌的谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)活力、羟基自由基清除能力和8株乳酸菌的总还原力、超氧阴离子自由基清除能力,分别筛选出抗氧化性强的产朊假丝酵母(酵母菌Y1)和干酪乳杆菌(乳酸菌L8)。比较了14种中药液态混菌(酵母菌Y1和乳酸菌L8)发酵前后酪氨酸酶抑制率和总还原力的变化,得出红景天为最佳发酵中药。以红景天为原料,采用纤维素酶-果胶酶酶解后添加其他营养成分,以酪氨酸酶抑制率为指标,采用响应面法优化混菌发酵红景天的条件:葡萄糖质量分数12.35%、液料比18.67∶1 (mL∶g)、发酵时间5.25 d,在此条件下红景天发酵液酪氨酸酶抑制率为73.21%,比未发酵的提高了67.72%。
Abstract:By determining the content of glutathione (GSH), SOD enzyme activity, and hydroxyl free radical scavenging ability of 12 yeast strains and total reducing capacity, and superoxide anion free radical scavenging ability of 8 lactic acid bacteria, Y1 (Candida utilis) and L8 (Lactobacillus casei) with high antioxidant activity were screened. The changes of tyrosinase inhibition rate and total reducing capacity of 14 kinds of Chinese traditional herb medicine with mixed bacteria (Y1 and L8) were compared before and after fermentation. Based upon test results, it is suggested that Rhodiola rosea (after fermentation) is the best Chinese traditional herb medicine to be used. Rhodiola rosea as the starting material is to be hydrolyzed by cellulase-pectinase complex enzyme; with tyrosinase inhibition rate as the index; the conditions for fermentation of Rhodiola rosea with mixed bacteria were optimized by using response surface methodology. Results indicate that the conditions of Rhodiola rosea fermented by mixed bacteria can be recommended as follows: glucose mass fraction, 12.35%; liquid-solid ratio, 18.67∶1 (mL∶g); fermentation time, 5.25 d. Under such conditions, the tyrosinase inhibition rate achieves 73.21%, which is 67.72% higher than that before the fermentation.
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