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日用化学工业:2017,Vol.>>Issue(1):36-39
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大鲵肽在牙膏中的应用研究
赵冠华,佟长青,李伟,曲敏
(大连海洋大学 食品科学与工程学院)
Research on applica¬tion of peptide from flesh of artificial raised Andrias davidianus in toothpaste
ZHAO Guan-hua,TONG Chang-qing,LI Wei,QU Min
(College of Food Science and Engineering, Dalian Ocean University)
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投稿时间:2016-08-18    
中文摘要: 采用酶法从人工饲养大鲵肉中提取得到大鲵肽,利用串联飞行时间质谱仪(MALDI-TOF/TOF)测定其分子量主要为1 422,1 521,1 746,1 828和1 879,均小于2 000,酶解产物大鲵肽为低聚肽。将提取到的大鲵肽应用到牙膏中,应用自由基清除活性评价方法研究了大鲵肽牙膏的抗氧化活性。结果表明,牙膏中添加质量分数为0.1%的大鲵肽可达到较好的抗氧化效果,其牙膏水溶液具有清除DPPH自由基、羟基自由基(OH)、超氧阴离子自由基以及ABTS自由基的能力。
中文关键词: 牙膏  大鲵肽  抗氧化
Abstract:A kind of peptide was isolated from flesh of artificial raised Andrias davidianus via enzymolysis. Molecular weight of the peptide was measured as 1 422, 1 521, 1 746, 1 828 and 1 879 with a tandem time-of flight MS system (MALDI-TOF/TOF). All the molecular weight values are lower than 2 000; and showed that the enzymolysis product is a kind of oligopeptide. The peptide extracted from Andrias davidianus was applied in formulation of toothpaste, and its antioxidation activity was examined based on method for determination of free radical scavenging activity. Results showed that when mass fraction of the peptide extract in the toothpaste formulation achieves 0.1%, rather good antioxidation effect can be obtained. Aqueous solution of the toothpaste is capable to scavenge DPPH free radicals, hydroxyl free radicals, superoxide anion radicals and ABTS free radi¬cals.
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