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22 August 2016, Volume 46 Issue 8 Previous Issue    Next Issue

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Study on the interaction of multi-quaternary ammonium salt with salmon sperm DNA LI Ya-ping,LAI Lu,ZHONG Zhou,MEI Ping,CHEN Wu,LIU Yi 2016, 46 (8):  427-432.  doi: 10.13218/j.cnki.csdc.2016.08.001 Abstract ( 275 )   PDF (2838KB) ( 289 )   The interaction between the multi-quaternary ammonium salt (MQAS) and salmon sperm DNA was investigated using UV-vis absorption spectroscopy,fluorescence spectroscopy,circular dichroism spectroscopy and molecular simulation.The results showed that MQAS binds to salmon sperm DNA by static electricity,and the binding ratio is 5.5∶1.The presence of salmon sperm DNA promotes the formation of MQAS aggregates.With decreasing chain length,the interaction between MQAS and salmon sperm DNA is stronger.In addition,MQAS causes the conformational change of salmon sperm DNA.The results of molecular simulation suggested that MQAS inserts into the minor groove of salmon sperm DNA,and forms stable compound with DNA. References | Related Articles | Metrics

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Fluorinated surfactants and fluoropolymers (Ⅷ) Progress in field of fluorinated fabric finishing agents and advice for its further development XING Hang,ZHOU Hong-tao,XIAO Jin-xin 2016, 46 (8):  433-440.  doi: 10.13218/j.cnki.csdc.2016.08.002 Abstract ( 273 )   PDF (846KB) ( 410 )   History and status of industrial production and commercial marketing of fluorinated fabric finishing agents in China domestic field and worldwide as well as development of substitutes for long carbon chain (mainly C8) fluorinated compounds were described.In view of environmental protection and cost consideration,the blend type fluorinated fabric finishing agents would have big space for potential development.Suggestions for further exploration in this field were put forward. References | Related Articles | Metrics

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Surface activities of double-tailed and multi-tailed fluorinated surfactants in organic solvents CHEN Xian-tao,XING Hang,WU Yi,XIAO Jin-xin 2016, 46 (8):  441-446.  doi: 10.13218/j.cnki.csdc.2016.08.003 Abstract ( 261 )   PDF (811KB) ( 338 )   This work attempts to reduce the surface tension of organic solvents with oil-soluble fluorinated surfactants.Number of fluorocarbon chains in the surfactant molecule is increased thus to enhance density of the fluorocarbon chains adsorbed on surface layer of the organic solvents.Two series of fluorinated surfactants were synthesized:through the reaction of perfluorooctane sulfonyl fluoride with diamine (NH2(CH2)nNH2,n=2,3,6,10),the oil-soluble fluorinated surfactants,containing sulfonyl amido group as the spacer,with two fluorocarbon chains in one surfactant molecule were obtained;through the reaction of perfluorooctanoic acid with polyhydric alcohols (glycol,1,2-propanediol,glycerol and pentaerythrite),the oil-soluble fluorinated surfactants,containing ester group as the spacer with two,three and four fluorocarbon chains in one surfactant molecule were obtained respectively.The surface activity of the two series of fluorinated surfactants in ethanol,ethyl acetate,butanone and the ternary mixed solvent of ethanol,ethyl acetate and butanone were studied.The effects of molecular structure of surfactants and the type of organic solvent on their surface activity were observed.The results showed that there was no simple inevitability between surface tension reduction and the number of fluorocarbon tails.The configuration of the whole molecule was also important,and the type of these polar organic solvents could have significant influences on the surface tension of such surfactant solution. References | Related Articles | Metrics

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Study on the properties of ester trimethoxy organosilicon quaternary ammonium salt CHEN Rui-lan,ZHENG Cheng,MAO Tao-yan,CHEN Peng,LI Min-shan,LIN Jing 2016, 46 (8):  447-449.  doi: 10.13218/j.cnki.csdc.2016.08.004 Abstract ( 292 )   PDF (1214KB) ( 270 )   The surface properties and biodegradation characteristics of the ester trimethoxy organosilicon quaternary ammonium salt (ETOQAS) were measured.The results showed that at 25 ℃,the critical micelle concentration (cmc) of ETOQAS measured by surface tension method is 0.01 g/L,and γcmc is 15.72 mN/m.Meanwhile,NaCl was added into ETOQAS aqueous solution,and its cmc,measured by steady-state fluorescence method shows decreasing.As the mass concentration of ETOQAS increases,the contact angle on surface of iron and paraffin wax decreases.ETOQAS can be completely biodegraded within 3 d. References | Related Articles | Metrics

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Hydraulic cavitation enhancement of coconut fatty-acylation of soybean meal protein CHENG Hai-tao,SHEN Xian-shuang 2016, 46 (8):  450-453.  doi: 10.13218/j.cnki.csdc.2016.08.005 Abstract ( 287 )   PDF (1987KB) ( 264 )   Hydraulic cavitation was used to strengthen reaction of modified soybean meal protein by coconut fatty acyl chloride.Factors that affecting the yield of the product such as pressure,time,temperature and material to liquid ratio (volume ratio of water soluble soybean meal solution to coconut fatty acyl chloride),were studied with a set of self-designed hydraulic cavitation equipment.Based on single factor experiments,the optimum conditions were obtained using response surface methodology and surface properties of acylated product were determined.The results showed that the optimal technological conditions for reaction of modified soybean meal protein by coconut fatty acyl chloride with hydraulic cavitation are as follows:pressure,0.29 MPa;time,49 min;temperature,52 ℃;material to liquid ratio,1.8.The product yield achieves 98.1%.The surface performance of the product is fine. References | Related Articles | Metrics

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Foaming performance of blend system of sodium fatty alcohol ether carboxylate and iso-fatty alcohol-polyoxyethylene ether LIU Xiao-ying,TAI Xiu-mei,DU Zhi-ping, MAO Xue-bin,ZHAO Hui-xian 2016, 46 (8):  454-457.  doi: 10.13218/j.cnki.csdc.2016.08.006 Abstract ( 293 )   PDF (833KB) ( 310 )   Foaming performance of the blend system of sodium fatty alcohol ether carboxylate (C12~14E9C) and iso-fatty alcohol-polyoxyethylene ether (IC10EO8) were measured by Ross-Miles method.The results showed that the blend system exhibits high foaming power,and the surface energy of the system is the lowest when the mass ratio of C12~14E9C to IC10EO8 is 6∶4 and the mass concentration is 2 g/L.As the mass concentration is further increasing,the foam stability of the blend system becomes declining obviously.The blend system exhibits strong resistance to hard water.Its foaming power and foam stability do not change when the water hardness is less than 400 mg/kg.Addition of salt shows no impact on the foaming power of the blend system,but 0.5% sodium chloride and trisodium citrate can reduce its foam stability.Foaming power of the blend system increases with the temperature increasing,while the foam stability declines.Foaming power of the blend system increases with pH increasing.When pH of the system is higher than 5,its foaming power remains basically unchanged;and when pH is within 3-9,no significant change exhibits on its foam stability. References | Related Articles | Metrics

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Effect of viscosity-average molecular weight of chitosan on its application as surface sizing agent WANG Zhi-jie,LI Yu-jie,WANG Jian 2016, 46 (8):  458-461.  doi: 10.13218/j.cnki.csdc.2016.08.007 Abstract ( 229 )   PDF (1592KB) ( 323 )   A series of chitosan of different viscosity-average molecular weight were prepared by oxidation of chitosan with hydrogen peroxide as the oxidizing agent.The chitosan of different viscosity-average molecular weight was applied as paper surface sizing agent,and the effect to the surface properties of the paper was studied.Results showed that the reducing of chitosan viscosity-average molecular weight is not beneficial to its surface sizing effect.Such result was confirmed by SEM.Under same conditions,as comparing with using oxidized starch alone,the mixture with a small amount of chitosan and oxidized starch can improves the surface strength of paper obviously. References | Related Articles | Metrics

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Synthesis of hierarchical EU-1 zeolite using quaternary ammonium salt modified starch assisted vapor phase method and its catalytic performance LIU Xiao-zhen,LI Xiao-feng,GAO Meng,GAO Li-xia,LIU Yong-mei,DOU Tao 2016, 46 (8):  462-466.  doi: 10.13218/j.cnki.csdc.2016.08.008 Abstract ( 256 )   PDF (1974KB) ( 313 )   Using quaternary ammonium salt modified starch as mesoporous template,hierarchical EU-1 zeolite was synthesized by vapor phase method.The physicochemical properties of the synthesized samples were characterized by XRD,FT-IR,SEM,N2 adsorption-desorption and NH3-TPD.The results showed that by regulating the dosage of quaternary ammonium salt modified starch,the mesoporous pore volume of the sample is increased by 0.081 cm3/g and the apparent surface area of the sample is increased by 23.35 cm2/g.The catalytic performance of the samples is examined by xylene isomerization test.The results showed that the equilibrium concentration of PX is increased by 0.88 percent points,the ethylbenzene conversion is increased by 8.47 percent points and the yield of C8 hydrocarbons is increased by 1.66 percent points. References | Related Articles | Metrics

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Cloning and expression characterization of transglucosidase gene for biological catalytic synthesis of α-arbutin WANG Yi-dong,WEI Pan-pan,SHAO Meng-ge,ZHANG Chao-hui,CHEN Xiao-long 2016, 46 (8):  467-472.  doi: 10.13218/j.cnki.csdc.2016.08.009 Abstract ( 257 )   PDF (3078KB) ( 293 )   The transglucosidase gene from Xanthomonas campestris pv.campestris was ligated with expression vector pET28a to construct plasmids pET28a-agl by using One Step Cloning Kit,transformed into different competent cells and screened out the resulting recombinants E.coli BL21 Gold (DE3) plysS pET28a-agl that successfully expresses the transglucosidase and its apparent high enzymatic activity for catalyzing enzymatic synthesis of α-arbutin.Enzymatic properties of the transglucosidase obtained were studied with IMAC affinity chromatograph.The suitable conditions for synthesis of α-arbutin are as follows:mass concentration of the transglucosidase,0.272 5 g/L;mass concentration of hydroquinone,11 g/L;concentration of maltose,1.1 mol/L;concentration of phosphate buffer solution,100 mmol/L (pH=6.5);shaking for 3 h at 30 ℃.It was discovered that the reaction product α-arbutin shows slight inhibition effect on the action of the transglucosidase.K+ ion of 1 mmol/L enhances the enzyme activity while Cu2+ of 1 mmol/L almostly inhibits the activity of the transglucosidase completely. References | Related Articles | Metrics

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Adverse Outcome Pathways Guide and toxicity test applications QU Xiao-ting,CHENG Shu-jun,QIN Yao,KE Yi-hui 2016, 46 (8):  473-478.  doi: 10.13218/j.cnki.csdc.2016.08.010 Abstract ( 414 )   PDF (1096KB) ( 366 )   The arising and development of Adverse Outcome Pathways (AOP) under the advocation of toxicity test vision and strategy in the 21st century as well as the pressure of regulation system changes and development of modern science and technology were reviewed.The concept and guide of AOP;the basic composition,structural conditions and information sharing of AOP as well as network and assessment of AOP were emphatically introduced.The development and application situation in two toxicity endpoints,the skin sensitization test and fish early-growth stage test were discussed as the practice instances of different AOP development strategy that is top-down approach and middle-out approach.Finally,it was concluded that the AOP relies on the development of modern science and technology as the driving force,toxicity prediction modernization as the basis,regulation mode change as the target.AOP guideline also provides an opportunity for the development of alternatives to animal testing method and testing technology innovation. References | Related Articles | Metrics

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Study of multiplex real-time fluorescent PCR detection method for animal originated components in cosmetics LIU Yan-yan,HUO Sheng-nan,LIANG Shui-mei,BIAN Ru-ru,ZHANG Hui,BU Xun 2016, 46 (8):  479-484.  doi: 10.13218/j.cnki.csdc.2016.08.011 Abstract ( 259 )   PDF (1650KB) ( 328 )   DNA of animal originated components in paste,liquid and solid form cosmetic products were sampled using improved Chelex-100 combined with Glass Milk method.According to the donkey,horse and cattle mitochondrial 16S rRNA gene sequences,the universal primers and specific probes were designed.Then a multiplex real-time fluorescent PCR assay system to detect the species of animal originated (donkey,horse and cattle) components in cosmetic products was established.All the detection limit of all cosmetics achieves 0.001 ng.Samples from marketed cosmetics such as creams,lotions and facial masks were detected by this way.The reliability and accuracy of this multiplex real-time fluorescent PCR assay were confirmed. References | Related Articles | Metrics

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Determination of octadecylamine hydrochloride in saturated salt solution using orange Ⅱ spectrophotometric method LIANG Hui-bin,DU Zhi-ping,LI En-ze,DUAN Ping-mei,CHENG Fang-qin 2016, 46 (8):  485-488.  doi: 10.13218/j.cnki.csdc.2016.08.012 Abstract ( 352 )   PDF (752KB) ( 364 )   Determination of content of octadecylamine hydrochloride in KCl saturated solution with orange Ⅱ spectrophotometric method was studied.Factors that affecting the test results such as the amount of buffer solution and indicator,and the time for stabilizing the chromogenic reaction were investigated;and the optimized experimental conditions were identified.Results indicated that the maximum absorption wavelength of the complex formed by octadecylamine hydrochloride and orange Ⅱ is 485 nm that showing good linearity with regression equation of y=0.063 7x-0.068 9.The correlation coefficient is 0.991 3.The relative standard deviation of quantitative analysis method is 1.53%. References | Related Articles | Metrics