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China Surfactant Detergent & Cosmetics ›› 2016, Vol. 46 ›› Issue (8): 467-472.doi: 10.13218/j.cnki.csdc.2016.08.009

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Cloning and expression characterization of transglucosidase gene for biological catalytic synthesis of α-arbutin

WANG Yi-dong,WEI Pan-pan,SHAO Meng-ge,ZHANG Chao-hui,CHEN Xiao-long   

  1. College of Biological Engineering,Zhejiang University of Technology,Hangzhou,Zhejiang 310014,China
  • Online:2016-08-22 Published:2019-04-18

Abstract: The transglucosidase gene from Xanthomonas campestris pv.campestris was ligated with expression vector pET28a to construct plasmids pET28a-agl by using One Step Cloning Kit,transformed into different competent cells and screened out the resulting recombinants E.coli BL21 Gold (DE3) plysS pET28a-agl that successfully expresses the transglucosidase and its apparent high enzymatic activity for catalyzing enzymatic synthesis of α-arbutin.Enzymatic properties of the transglucosidase obtained were studied with IMAC affinity chromatograph.The suitable conditions for synthesis of α-arbutin are as follows:mass concentration of the transglucosidase,0.272 5 g/L;mass concentration of hydroquinone,11 g/L;concentration of maltose,1.1 mol/L;concentration of phosphate buffer solution,100 mmol/L (pH=6.5);shaking for 3 h at 30 ℃.It was discovered that the reaction product α-arbutin shows slight inhibition effect on the action of the transglucosidase.K+ ion of 1 mmol/L enhances the enzyme activity while Cu2+ of 1 mmol/L almostly inhibits the activity of the transglucosidase completely.

Key words: additive of cosmetics, α-arbutin, biocatalysis

CLC Number: 

  • TQ658